Western Blot (WB)
(Western Blot analysis of Human A431 and HEK293 cell lysates showing detection of TCP1 alpha protein using Rat Anti-TCP1 alpha Monoclonal Antibody, Clone 23c. Primary Antibody: Rat Anti-TCP1 alpha Monoclonal Antibody at 1:1000.)
Rat TCP1-alpha Monoclonal Antibody | anti-Tcp1 antibody
TCP1 alpha Antibody: HRP
Western Blot (WB)
(Western Blot analysis of Human A431 and HEK293 cell lysates showing detection of TCP1 alpha protein using Rat Anti-TCP1 alpha Monoclonal Antibody, Clone 23c. Primary Antibody: Rat Anti-TCP1 alpha Monoclonal Antibody at 1:1000.)
Western Blot (WB)
(Western Blot analysis of Human A431 and HEK293 cell lysates showing detection of TCP1 alpha protein using Rat Anti-TCP1 alpha Monoclonal Antibody, Clone 23c. Primary Antibody: Rat Anti-TCP1 alpha Monoclonal Antibody at 1:1000.)
Scientific Background: T-complex polypeptide-1 (TCP1) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is up-regulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. The TCP1 sequence shows nearly 40% identity to TF55, but only minimal similarity to HSP60 and GroEL.
NCBI and Uniprot Product Information
Uniprot Description
CCT-alpha: Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter. Interacts with PACRG. Component of the BBS/CCT complex composed at least of MKKS, BBS10, BBS12, TCP1, CCT2, CCT3, CCT4, CCT5 AND CCT8. Belongs to the TCP-1 chaperonin family.
Protein type: Motility/polarity/chemotaxis; Chaperone
Cellular Component: Golgi apparatus; chaperonin-containing T-complex; heterochromatin; microtubule; centrosome; pericentriolar material; cytoskeleton; cytoplasm; acrosome; microtubule organizing center; nuclear heterochromatin; myelin sheath
Molecular Function: protein binding; ubiquitin protein ligase binding; unfolded protein binding; nucleotide binding; ATP binding
Biological Process: protein folding; binding of sperm to zona pellucida
Research Articles on Tcp1
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Product Notes
The Tcp1 tcp1 (Catalog #AAA802751) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The TCP1 alpha Antibody: HRP reacts with Mouse, Rat, Sheep, Rabbit, Hamster, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's TCP1-alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). 1:1000 (WB). Researchers should empirically determine the suitability of the Tcp1 tcp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCP1-alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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