Rabbit anti-V5 tag V5 Polyclonal Antibody | anti-V5 antibody

Anti-V5 tag Antibody, Rabbit Polyclonal

Cat. Num. AAA8100289

• Reactivity: V5 tag
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: V5; Polyclonal Antibody; Anti-V5 tag Antibody; Rabbit Polyclonal; Anti-V5 Tag Antibody; Rabbit PAb; Antigen Affinity Purified; anti-V5 antibody

• Host: Rabbit
• Reactivity: V5 tag
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Recognize N-terminal and C-terminal V5 Tag in fusion proteins.
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-V5 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:1000-1:5000; IP: 1-4ul/mg of lysate
• Immunogen: A synthetic peptide corresponding to the V5 tag sequence
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, a synthetic peptide corresponding to the V5-tag sequence. V5-tag specific IgG was purified by V5-taq affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(V5-tag was immunoprecipitated using: Lane A:0.5 mg his-ARG1-V5 Transfected 293 Cell Lysate Lane B:0.5 mg V5-ARG1-his Transfected 293 Cell Lysate Lane C:0.5 mg mFABP4-V5-his Transfected 293 Cell Lysate Lane D:0.5 mg V5-mFABP4-his Transfected 293 Cell Lysate Lane E:0.5 mg Negative control 4 uL anti-V5-tag rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-V5-tag rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution Developed using the Odyssey technique. Performed under reducing conditions.)

Western Blot (WB)

(Anti-V5 Tag rabbit polyclonal antibody at 1:1000 dilution Sample:Recombinant Protein Lane A: V5-mFABP4-his(30ng) Lane B: his-ARG1-V5(30ng) Lane C: mFABP4-V5-his(30ng) Lane D:Negative control Secondary Dylight 800-labeled Antibody To Rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions.)

Western Blot (WB)

(Anti-V5 Tag rabbit polyclonal antibody at 1:1000 dilution Lane A: his-ARG1-V5 Transfected 293 Cell Lysate Lane B: V5-ARG1-his Transfected 2933 Cell Lysate Lane C: V5-mFABP4-his Transfected 293 Cell Lysate Lane D: mFABP4-V5-his Transfected 293 Cell Lysate Lane E: Negative control Lysates/proteins at 2 ug per Lane. Secondary Dylight 800-labeled Antibody To Rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions.)

Related Product Information for anti-V5 antibody

The V5 epitope tag has a 14-amino-acid sequence (GKPIPNPLLGLDST) which is derived from simian parainfluenza virus type 5 (SV5). The V5 epitope tag is used to label a protein of interest so that the tagged protein can be analyzed and visualized. Anti-V5 epitope tag antibody products recognize and detect V5-tagged proteins without a protein-specific antibody or probe in several immunochemical assays such as Western Blotting, ELISAs and immunostaining techniques.

References

Zhao X, et al. (2013) Several affinity tags commonly used in chromatographic purification. J Anal Methods Chem 2013 581093.Monti M, et al. (2007) Functional proteomics: Protein-protein interactions in vivo. Ital J Biochem 56 (4): 310-314.Brothers SP, et al. (2003) Unexpected effects of epitope and chimeric tags on gonadotropin-releasing hormone receptors: Implications for understanding the molecular etiology of hypogonadotropic hypogonadism. J Clin Endocrinol Metab 88 (12): 6107-6112.