Immunoprecipitation (IP)
(STX8 was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell Lysate4 uL anti-STX8 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-STX8 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 27 kDaObserved band size: 32 kDa)
Rabbit Syntaxin 8 Polyclonal Antibody | anti-STX8 antibody
Anti-Syntaxin 8 Antibody, Rabbit Polyclonal
Gene Names
STX8; CARB
Reactivity
HumanPredicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Applications
Western Blot, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
Syntaxin 8; Polyclonal Antibody; Anti-Syntaxin 8 Antibody; Rabbit Polyclonal; STX8/Syntaxin 8 Antibody; Rabbit PAb; Antigen Affinity Purified; syntaxin 8; Anti-CARB Antibody; anti-STX8 antibody
Host
Rabbit
Reactivity
Human
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human Syntaxin 8
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-STX8 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IP: 1-4uL/mg of lysate
IP: 1-4uL/mg of lysate
Immunogen
A synthetic peptide corresponding to the center region of the Human STX8/Syntaxin 8
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human STX8/Syntaxin 8, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunoprecipitation (IP)
(STX8 was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell Lysate4 uL anti-STX8 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-STX8 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 27 kDaObserved band size: 32 kDa)
Immunoprecipitation (IP)
(STX8 was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell Lysate4 uL anti-STX8 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-STX8 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 27 kDaObserved band size: 32 kDa)
Western Blot (WB)
(Anti-STX8 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:27 kDaObserved band size:27 kDa)
Western Blot (WB)
(Anti-STX8 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:27 kDaObserved band size:27 kDa)
Related Product Information for anti-STX8 antibody
STX8, also known as syntaxin 8, directly interacts with HECTd3. STX8 forms the SNARE complex with syntaxin 7, vti1b and endobrevin. STX8 belongs to the syntaxin family. Members of this family are key molecules implicated in diverse vesicle docking and membrane fusion events. STX8 physically interacts with cystic fibrosis transmembrane conductance regulator (CFTR): recombinant syntaxin 8 binds CFTR in vitro and both proteins co-immunoprecipitate in HT29 cells. Syntaxin 8 regulates CFTR-mediated currents in chinese hamster ovary (CHO) cells stably expressing CFTR and syntaxin 8. STX8 contributes to the regulation of CFTR trafficking and chloride channel activity by the SNARE machinery.
References
Steegmaier M. et al., 1999, J Biol Chem. 273 (51): 34171-9. Thoreau V. et al., 1999, Biochem Biophys Res Commun. 257 (2): 577-83. Zhang L. et al., 2009, Cell Mol Neurobiol. 29 (1): 115-21. Bilan F. et al., 2004, J Cell Sci. 117 (10): 1923-35.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
26,907 Da
NCBI Official Full Name
syntaxin 8
NCBI Official Synonym Full Names
syntaxin 8
NCBI Official Symbol
STX8
NCBI Official Synonym Symbols
CARB
NCBI Protein Information
syntaxin-8
UniProt Protein Name
Syntaxin-8
Protein Family
UniProt Gene Name
STX8
NCBI Description
The gene is a member of the syntaxin family. The encoded protein is involved in protein trafficking from early to late endosomes via vesicle fusion and exocytosis. A related pseudogene has been identified on chromosome 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]
Uniprot Description
Vesicle trafficking protein that functions in the early secretory pathway, possibly by mediating retrograde transport from cis-Golgi membranes to the ER.
Research Articles on STX8
Similar Products
Product Notes
The STX8 stx8 (Catalog #AAA8100867) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Syntaxin 8 Antibody, Rabbit Polyclonal reacts with Human Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's Syntaxin 8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:2000 IP: 1-4uL/mg of lysate. Researchers should empirically determine the suitability of the STX8 stx8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Syntaxin 8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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