Western Blot (WB)
(Lanes:1:non-transfected cells;2:V5-tagged empty plasmid;3:protein A (V5-tagged);4:protein B (V5-tagged);5:protein C (V5-tagged);6:protein D (V5-tagged);Protocol and data courtesy of Dr. Gustavo Gutierrez, Burnham Institute for Medical Research.)
Rabbit V5 Tag Polyclonal Antibody
V5 Tag Antibody
Applications
Western Blot, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
V5 Tag; Polyclonal Antibody; V5 Tag Antibody; Tag from influenza hemagglutinin protein; anti-V5 Tag antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
KLH conjugated synthetic peptide encoding the V5 tag (GKPIPNPLLGLDST) was used as antigen.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Applicable Applications for anti-V5 Tag antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Lanes:1:non-transfected cells;2:V5-tagged empty plasmid;3:protein A (V5-tagged);4:protein B (V5-tagged);5:protein C (V5-tagged);6:protein D (V5-tagged);Protocol and data courtesy of Dr. Gustavo Gutierrez, Burnham Institute for Medical Research.)
Western Blot (WB)
(Lanes:1:non-transfected cells;2:V5-tagged empty plasmid;3:protein A (V5-tagged);4:protein B (V5-tagged);5:protein C (V5-tagged);6:protein D (V5-tagged);Protocol and data courtesy of Dr. Gustavo Gutierrez, Burnham Institute for Medical Research.)
Western Blot (WB)
(Western blot analysis of anti-V5 Tag Antibody in 12tag protein (35ug/lane). 12 Tag (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-V5 Tag Antibody in 12tag protein (35ug/lane). 12 Tag (arrow) was detected using the purified Pab.)
Related Product Information for anti-V5 Tag antibody
Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The V5 epitope tag is is composed of a 14 residue peptide, GKPIPNPLLGLDST, derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). MyBioSource's anti-V5 tag polyclonal antibody provides a simple solution to detect the expression of a V5-tagged protein in cells.
Product Categories/Family for anti-V5 Tag antibody
References
Dunn C, et al.J Immunol Methods. 1999 Apr 22;224(1-2):141-50.
Similar Products
Product Notes
The V5 Tag (Catalog #AAA9205957) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's V5 Tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the V5 Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "V5 Tag, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
