Immunoprecipitation (IP)
(HA tag was immunoprecipitated using: Lane A:0.5 mg HA-ARG1-myc transfected 293 Whole Cell Lysate Lane B:0.5 mg myc-ARG1-HA transfected 293 Whole Cell Lysate 2 uL anti-HA tag mouse monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-HA tag mouse monoclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 39 kDa Observed band size: 39 kDa)
Mouse anti-HA tag HA Monoclonal Antibody | anti-HA antibody
Anti-HA tag Antibody, Mouse Monoclonal
Reactivity
HA tag
Applications
Immunoprecipitation
Purity
Protein A
Synonyms
HA; Monoclonal Antibody; Anti-HA tag Antibody; Mouse Monoclonal; Anti-HA Tag Antibody; Mouse MAb; anti-HA antibody
Host
Mouse
Reactivity
HA tag
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
15
Specificity
Recognize N-terminal and C-terminal HA Tag in fusion proteins.
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-HA antibody
Immunoprecipitation (IP)
Application Notes
IP: 4-8uL/mg of lysate
Immunogen
A synthetic peptide corresponding to the HA-tag sequence (YPYDVPDYA)
Conjugation
Unconjugated
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, a synthetic peptide corresponding to the HA-tag sequence. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunoprecipitation (IP)
(HA tag was immunoprecipitated using: Lane A:0.5 mg HA-ARG1-myc transfected 293 Whole Cell Lysate Lane B:0.5 mg myc-ARG1-HA transfected 293 Whole Cell Lysate 2 uL anti-HA tag mouse monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-HA tag mouse monoclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 39 kDa Observed band size: 39 kDa)
Immunoprecipitation (IP)
(HA tag was immunoprecipitated using: Lane A:0.5 mg HA-ARG1-myc transfected 293 Whole Cell Lysate Lane B:0.5 mg myc-ARG1-HA transfected 293 Whole Cell Lysate 2 uL anti-HA tag mouse monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-HA tag mouse monoclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 39 kDa Observed band size: 39 kDa)
Related Product Information for anti-HA antibody
The human influenza hemagglutinin (HA) epitope was derived from the protein on the HA virus. HA tag is the amino acid sequence of a YPYDVPDYA tag and a particularly common tags. HA is tagged onto proteins for study and analysis and often used in fusion protein-related research through the realization of detection of HA-tag for the detection of fusion proteins. Antibodies to HA-tag are the key of the most important tools in the detection and often used in purification of HA tag-related proteins, study of the protein interaction and detection of target protein expression.
References
Zhao X, et al. (2013) Several affinity tags commonly used in chromatographic purification. J Anal Methods Chem 2013 581093.Monti M, et al. (2007) Functional proteomics: Protein-protein interactions in vivo. Ital J Biochem 56 (4): 310-314.Brothers SP, et al. (2003) Unexpected effects of epitope and chimeric tags on gonadotropin-releasing hormone receptors: Implications for understanding the molecular etiology of hypogonadotropic hypogonadism. J Clin Endocrinol Metab 88 (12): 6107-6112.
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Product Notes
The HA (Catalog #AAA8100026) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-HA tag Antibody, Mouse Monoclonal reacts with HA tag and may cross-react with other species as described in the data sheet. AAA Biotech's HA can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP). IP: 4-8uL/mg of lysate. Researchers should empirically determine the suitability of the HA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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