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Western Blot (WB) (Western blot analysis of TRPV1 in K562 cell lysate with TRPV1 antibody at 1 μg/mL.)

Rabbit anti-Human TRPV1 Polyclonal Antibody | anti-TRPV1 antibody

TRPV1 Antibody

Gene Names
TRPV1; VR1
Reactivity
Human
Applications
ELISA, Western Blot
Purity
TRPV1 Antibody is affinity chromatography purified via peptide column.
Synonyms
TRPV1; Polyclonal Antibody; TRPV1 Antibody; VR1; Transient receptor potential cation channel subfamily V member 1; Capsaicin receptor; TrpV1; transient receptor potential cation channel; subfamily V; member 1; anti-TRPV1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
TRPV1 Antibody is affinity chromatography purified via peptide column.
Form/Format
TRPV1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration
1 mg/mL (varies by lot)
Sequence Length
839
Applicable Applications for anti-TRPV1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
TRPV1 antibody can be used for detection of TRPV1 by Western blot at 1 - 2 ug/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Optimal dilutions for each application to be determined by the researcher.
Homology
Predicted species reactivity based on immunogen sequence: Guinea pig: (81%), Mouse: (75%)
Immunogen
Rabbit polyclonal TRPV1 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human TRPV1. The immunogen is located within the last 50 amino acids of TRPV1.
Positive Control
1) Cat# MBS151739 - K562 Cell Lysate
Conjugate
Unconjugated
Physical State
Liquid
Preparation and Storage
TRPV1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Western Blot (WB)

(Western blot analysis of TRPV1 in K562 cell lysate with TRPV1 antibody at 1 μg/mL.)

Western Blot (WB) (Western blot analysis of TRPV1 in K562 cell lysate with TRPV1 antibody at 1 μg/mL.)
Related Product Information for anti-TRPV1 antibody
TRPV1 Antibody: TRPV1 is a receptor for capsaicin, the main pungent ingredient in hot chili peppers, and elicits a sensation of burning pain by selectively activating sensory neurons. TRPV1 is a non-selective cation channel that is structurally related to members of the TRP family of ion channels such as TRPC3 and TRPC6. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted: 92 kDa
NCBI Official Full Name
transient receptor potential cation channel subfamily V member 1
NCBI Official Synonym Full Names
transient receptor potential cation channel, subfamily V, member 1
NCBI Official Symbol
TRPV1
NCBI Official Synonym Symbols
VR1
NCBI Protein Information
transient receptor potential cation channel subfamily V member 1; OTRPC1; capsaicin receptor; osm-9-like TRP channel 1; vanilloid receptor subtype 1; transient receptor potential vanilloid 1a; transient receptor potential vanilloid 1b
UniProt Protein Name
Transient receptor potential cation channel subfamily V member 1
UniProt Gene Name
TRPV1
UniProt Synonym Gene Names
VR1; TrpV1; OTRPC1
UniProt Entry Name
TRPV1_HUMAN

NCBI Description

Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TRPV1: a receptor for capsaicin and a non-selective cation channel that is structurally related to members of the TRP family of ion channels. Activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo.

Protein type: Channel, cation; Membrane protein, multi-pass; Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 17p13.2

Cellular Component: postsynaptic membrane; integral to plasma membrane; integral to membrane; plasma membrane; cell junction; intrinsic to plasma membrane

Molecular Function: calmodulin binding; transmembrane receptor activity; excitatory extracellular ligand-gated ion channel activity; calcium-release channel activity; calcium channel activity; phosphoprotein binding; ATP binding

Biological Process: cell surface receptor linked signal transduction; transport; chemosensory behavior; thermoception; transmembrane transport

Research Articles on TRPV1

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Product Notes

The TRPV1 trpv1 (Catalog #AAA153345) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRPV1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRPV1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). TRPV1 antibody can be used for detection of TRPV1 by Western blot at 1 - 2 ug/mL. Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application to be determined by the researcher. Researchers should empirically determine the suitability of the TRPV1 trpv1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRPV1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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