Western Blot (WB)
(Western Blot analysis of extracts of various cells, using UBE2O antibody.)
Rabbit anti-Human, Mouse UBE2O Polyclonal Antibody | anti-UBE2O antibody
UBE2O Polyclonal Antibody
Gene Names
UBE2O; E2-230K
Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
UBE2O; Polyclonal Antibody; UBE2O Polyclonal Antibody; KIAA1734; Ubiquitin-protein ligase O; (E3-independent) E2 ubiquitin-conjugating enzyme; anti-UBE2O antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
UBE2O polyclonal antibody detects endogenous levels of UBE2O protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
1292
Applicable Applications for anti-UBE2O antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:1000-1:4000
IP: 1:50-1:200
IP: 1:50-1:200
Immunogen
Recombinant protein of human UBE2O.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot analysis of extracts of various cells, using UBE2O antibody.)
Western Blot (WB)
(Western Blot analysis of extracts of various cells, using UBE2O antibody.)
Western Blot (WB)
(Western Blot analysis of extracts from normal (control) and UBE2O knockout (KO) HeLa cells, using UBE2O antibody.)
Western Blot (WB)
(Western Blot analysis of extracts from normal (control) and UBE2O knockout (KO) HeLa cells, using UBE2O antibody.)
Immunoprecipitation (IP)
(Immunoprecipitation analysis of 200ug extracts of A-431 cells using 3ug UBE2O antibody.)
Immunoprecipitation (IP)
(Immunoprecipitation analysis of 200ug extracts of A-431 cells using 3ug UBE2O antibody.)
Related Product Information for anti-UBE2O antibody
UBE20 (ubiquitin-conjugating enzyme E2O), also known as E2-230K, is a 1, 292 amino acid member of the uniquitin-conjugating enzyme family that is involved in protein modification. Expressed predominately in heart and skeletal muscle, UBE2O functions to catalyze the ATP-dependent covalent attachment of ubiquitin to select proteins, thereby targeting the ubiquitinated proteins for proteasomal degradation. The gene encoding UBE2O maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1, 200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 200kDa
NCBI Official Full Name
(E3-independent) E2 ubiquitin-conjugating enzyme
NCBI Official Synonym Full Names
ubiquitin conjugating enzyme E2 O
NCBI Official Symbol
UBE2O
NCBI Official Synonym Symbols
E2-230K
NCBI Protein Information
(E3-independent) E2 ubiquitin-conjugating enzyme
UniProt Protein Name
E2/E3 hybrid ubiquitin-protein ligase UBE2O
Protein Family
UniProt Gene Name
UBE2O
UniProt Synonym Gene Names
KIAA1734; Ubiquitin-conjugating enzyme E2-230K
UniProt Entry Name
UBE2O_HUMAN
Uniprot Description
UBE2O: Catalyzes the covalent attachment of ubiquitin to other proteins. Belongs to the ubiquitin-conjugating enzyme family.
Protein type: EC 6.3.2.19; Ubiquitin conjugating system; Ubiquitin ligase; Ligase
Chromosomal Location of Human Ortholog: 17q25.1
Cellular Component: nucleoplasm; cytoplasm; nucleus
Molecular Function: protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity
Biological Process: protein monoubiquitination; retrograde transport, endosome to Golgi; positive regulation of BMP signaling pathway
Research Articles on UBE2O
Similar Products
Product Notes
The UBE2O ube2o (Catalog #AAA3008798) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UBE2O Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's UBE2O can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:1000-1:4000 IP: 1:50-1:200. Researchers should empirically determine the suitability of the UBE2O ube2o for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE2O, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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