Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
UBE2O recombinant protein
UBE2O, Active
Gene Names
UBE2O; E2-230K
Applications
Western Blot
Synonyms
UBE2O; Active; E2-230K; FLJ12878; KIAA1734; UBE2O recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant proteins stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Applicable Applications for UBE2O recombinant protein
Ubiquitin Enzyme Assay, Western Blot (WB)
Tag
GST
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for UBE2O recombinant protein
Recombinant full-length human UBE2O was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The UBE2O gene accession number is BC022237.
UBE2O or ubiquitin-conjugating enzyme E2O is a part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin. HIV-1 Tat has been identified to have a physical interaction with ubiquitin-conjugating enzyme E2O (UBE2O) in human HEK293 and Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses.
UBE2O or ubiquitin-conjugating enzyme E2O is a part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin. HIV-1 Tat has been identified to have a physical interaction with ubiquitin-conjugating enzyme E2O (UBE2O) in human HEK293 and Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses.
Product Categories/Family for UBE2O recombinant protein
References
1. Mondal, S. et al: A bioluminescent assay for monitoring conjugation of ubiquitin and ubiquitin-like proteins. Anal. Biochem. 510: 41-51, 2016
2. Zhang, X. et al: Fine-tuning BMP7 signalling in adipogenesis by UBE2O/E2-230K-mediated monoubiquitination of SMAD6. EMBO J. 32(7):996-1007, 2013
3. Mashtalir, N. et al: Autodeubiquitination protects the tumor suppressor BAP1 from cytoplasmic sequestration mediated by the atypical ubiquitin ligase UBE2O. Mol. Cell. 54(3):392-406, 2014
2. Zhang, X. et al: Fine-tuning BMP7 signalling in adipogenesis by UBE2O/E2-230K-mediated monoubiquitination of SMAD6. EMBO J. 32(7):996-1007, 2013
3. Mashtalir, N. et al: Autodeubiquitination protects the tumor suppressor BAP1 from cytoplasmic sequestration mediated by the atypical ubiquitin ligase UBE2O. Mol. Cell. 54(3):392-406, 2014
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
~90 kDa
NCBI Official Full Name
Homo sapiens ubiquitin-conjugating enzyme E2O, mRNA
NCBI Official Synonym Full Names
ubiquitin-conjugating enzyme E2O
NCBI Official Symbol
UBE2O
NCBI Official Synonym Symbols
E2-230K
NCBI Protein Information
ubiquitin-conjugating enzyme E2 O; ubiquitin-protein ligase O; ubiquitin carrier protein O; ubiquitin-conjugating enzyme E2-230K; ubiquitin-conjugating enzyme E2 of 230 kDa
UniProt Protein Name
E2/E3 hybrid ubiquitin-protein ligase UBE2O
Protein Family
UniProt Gene Name
UBE2O
UniProt Synonym Gene Names
KIAA1734; Ubiquitin-conjugating enzyme E2-230K
UniProt Entry Name
UBE2O_HUMAN
Uniprot Description
UBE2O: Catalyzes the covalent attachment of ubiquitin to other proteins. Belongs to the ubiquitin-conjugating enzyme family.
Protein type: EC 6.3.2.19; Ubiquitin conjugating system; Ubiquitin ligase; Ligase
Chromosomal Location of Human Ortholog: 17q25.1
Cellular Component: nucleoplasm; cytoplasm; nucleus
Molecular Function: protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity
Biological Process: protein monoubiquitination; retrograde transport, endosome to Golgi; positive regulation of BMP signaling pathway
Research Articles on UBE2O
Similar Products
Product Notes
The UBE2O ube2o (Catalog #AAA517756) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's UBE2O can be used in a range of immunoassay formats including, but not limited to, Ubiquitin Enzyme Assay, Western Blot (WB). Researchers should empirically determine the suitability of the UBE2O ube2o for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE2O, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
