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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using UBE2O antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)

Rabbit anti-Human, Mouse UBE2O Polyclonal Antibody | anti-UBE2O antibody

UBE2O Polyclonal Antibody

Gene Names
UBE2O; E2-230K
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Affinity purification
Synonyms
UBE2O; Polyclonal Antibody; UBE2O Polyclonal Antibody; anti-UBE2O antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
1292
Applicable Applications for anti-UBE2O antibody
Western Blot (WB)
Application Notes
WB: 1:1000 - 1:4000
Immunogen
Recombinant protein of human UBE2O
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using UBE2O antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using UBE2O antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
Product Categories/Family for anti-UBE2O antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 141kDa
Molecular Weight: 1292
NCBI Official Full Name
(E3-independent) E2 ubiquitin-conjugating enzyme
NCBI Official Synonym Full Names
ubiquitin conjugating enzyme E2 O
NCBI Official Symbol
UBE2O
NCBI Official Synonym Symbols
E2-230K
NCBI Protein Information
(E3-independent) E2 ubiquitin-conjugating enzyme
UniProt Protein Name
(E3-independent) E2 ubiquitin-conjugating enzyme
UniProt Gene Name
UBE2O
UniProt Synonym Gene Names
KIAA1734; Ubiquitin-conjugating enzyme E2-230K
UniProt Entry Name
UBE2O_HUMAN

Uniprot Description

UBE2O: Catalyzes the covalent attachment of ubiquitin to other proteins. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: EC 6.3.2.19; Ubiquitin ligase; Ubiquitin conjugating system; Ligase

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: cytoplasm; nucleus

Molecular Function: ATP binding; protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity

Biological Process: positive regulation of BMP signaling pathway; protein monoubiquitination; retrograde transport, endosome to Golgi

Research Articles on UBE2O

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Product Notes

The UBE2O ube2o (Catalog #AAA9130454) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UBE2O Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's UBE2O can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000 - 1:4000. Researchers should empirically determine the suitability of the UBE2O ube2o for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE2O, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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