Western Blot (WB)
(Western blotAll lanes: UBE2O antibody at 0.8ug/mlLane 1: Hela whole cell lysateLane 2: A431 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 141 kDaObserved band size: 141 kDa)
Rabbit anti-Human UBE2O Polyclonal Antibody | anti-UBE2O antibody
UBE2O Antibody
Gene Names
UBE2O; E2-230K
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity Purified
Synonyms
UBE2O; Polyclonal Antibody; UBE2O Antibody; (E3-independent) E2 ubiquitin-conjugating enzyme (EC:2.3.2.242 Publications; KIAA1734; E2/E3 hybrid ubiquitin-protein ligase UBE2O; Ubiquitin carrier protein O; Ubiquitin-conjugating enzyme E2 O; Ubiquitin-conjugating enzyme E2 of 230 kDa; Ubiquitin-conjugating enzyme E2-230K; Ubiquitin-protein ligase O; anti-UBE2O antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Liquid
Sequence Length
1292
Applicable Applications for anti-UBE2O antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Recommended dilution: WB:1:500-2000
Immunogen
Recombinant human (E3-independent) E2 ubiquitin-conjugating enzyme protein
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-109125
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.
Western Blot (WB)
(Western blotAll lanes: UBE2O antibody at 0.8ug/mlLane 1: Hela whole cell lysateLane 2: A431 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 141 kDaObserved band size: 141 kDa)
Western Blot (WB)
(Western blotAll lanes: UBE2O antibody at 0.8ug/mlLane 1: Hela whole cell lysateLane 2: A431 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 141 kDaObserved band size: 141 kDa)
Related Product Information for anti-UBE2O antibody
E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins (PubMed:23455153, PubMed:24703950). Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity (PubMed:23381138). Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis (PubMed:23455153). Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location (PubMed:24703950). Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASH1, leading to promote endosomal F-actin assembly (PubMed:23452853).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
141,293 Da
NCBI Official Full Name
(E3-independent) E2 ubiquitin-conjugating enzyme
NCBI Official Synonym Full Names
ubiquitin conjugating enzyme E2O
NCBI Official Symbol
UBE2O
NCBI Official Synonym Symbols
E2-230K
NCBI Protein Information
(E3-independent) E2 ubiquitin-conjugating enzyme
UniProt Protein Name
(E3-independent) E2 ubiquitin-conjugating enzyme
Protein Family
UniProt Gene Name
UBE2O
UniProt Synonym Gene Names
KIAA1734; Ubiquitin-conjugating enzyme E2-230K
UniProt Entry Name
UBE2O_HUMAN
Uniprot Description
UBE2O: Catalyzes the covalent attachment of ubiquitin to other proteins. Belongs to the ubiquitin-conjugating enzyme family.
Protein type: Ubiquitin conjugating system; Ligase; EC 6.3.2.19; Ubiquitin ligase
Chromosomal Location of Human Ortholog: 17q25.1
Cellular Component: cytoplasm; nucleus
Molecular Function: ATP binding; protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity
Biological Process: positive regulation of BMP signaling pathway; protein monoubiquitination; retrograde transport, endosome to Golgi
Research Articles on UBE2O
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Product Notes
The UBE2O ube2o (Catalog #AAA7005326) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UBE2O Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's UBE2O can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Recommended dilution: WB:1:500-2000. Researchers should empirically determine the suitability of the UBE2O ube2o for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE2O, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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