Immunohistochemistry (IHC)
(MBS9603719 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit TRPC4AP Polyclonal Antibody | anti-TRPC4AP antibody
TRPC4AP Antibody
Gene Names
TRPC4AP; TRUSS; TRRP4AP; PPP1R158; C20orf188
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
TRPC4AP; Polyclonal Antibody; TRPC4AP Antibody; C20orf188; Chromosome 20 open reading frame 188; Protein TAP1; Protein TRUSS; Short transient receptor potential channel 4 associated protein; Short transient receptor potential channel 4-associated protein; TAP1 protein; TNF receptor ubiquitous scaffolding/signaling protein; TNF-receptor ubiquitous scaffolding/signaling protein; TP4AP_HUMAN; Transient receptor potential cation channel; subfamily C; member 4 associated protein; Trp4 associated protein; Trp4-associated protein; Trpc4 associated protein; Trpc4-associated protein; TRRP4AP; TRUSS; Tumor necrosis factor receptor-associated ubiquitous scaffolding and signaling protein; anti-TRPC4AP antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
TRPC4AP antibody detects endogenous levels of TRPC4AP.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
797
Applicable Applications for anti-TRPC4AP antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human TRPC4AP.
Predicted Cross Reactivity
Bovine, Horse, Sheep, Dog
Similarity
Bovine (100%), Horse (100%), Sheep (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9603719 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603719 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of extracts of human heart tissue, using TRPC4AP antibody.)
Western Blot (WB)
(Western blot analysis of extracts of human heart tissue, using TRPC4AP antibody.)
Related Product Information for anti-TRPC4AP antibody
Function: Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. The DCX(TRUSS) complex specifically mediates the polyubiquitination and subsequent degradation of MYC. Also participates in the activation of NFKB1 in response to ligation of TNFRSF1A, possibly by linking TNFRSF1A to the IKK signalosome. Involved in JNK activation via its interaction with TRAF2. Also involved in elevation of endoplasmic reticulum Ca2+ storage reduction in response to CHRM1.
Subunit Structure: Constitutively associated with TNFRSF1A. Directly interacts with TRADD, TRAF2, CHUK, IKBKB and IKBKG. Interacts with TRPC1, TRPC4 and TRPC5 (By similarity). Component of the DCX(TRUSS) E3 ubiquitin ligase complex, at least composed of CUL4A, DDB1, TRPC4AP/TRUSS and RBX1. Interacts directly with DDB1. Interacts with MYC.
Subunit Structure: Constitutively associated with TNFRSF1A. Directly interacts with TRADD, TRAF2, CHUK, IKBKB and IKBKG. Interacts with TRPC1, TRPC4 and TRPC5 (By similarity). Component of the DCX(TRUSS) E3 ubiquitin ligase complex, at least composed of CUL4A, DDB1, TRPC4AP/TRUSS and RBX1. Interacts directly with DDB1. Interacts with MYC.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 91 kDa
Predicted: 91 kDa
Predicted: 91 kDa
NCBI Official Full Name
short transient receptor potential channel 4-associated protein isoform a
NCBI Official Synonym Full Names
transient receptor potential cation channel subfamily C member 4 associated protein
NCBI Official Symbol
TRPC4AP
NCBI Official Synonym Symbols
TRUSS; TRRP4AP; PPP1R158; C20orf188
NCBI Protein Information
short transient receptor potential channel 4-associated protein
UniProt Protein Name
Short transient receptor potential channel 4-associated protein
UniProt Gene Name
TRPC4AP
UniProt Synonym Gene Names
C20orf188; TRRP4AP; Trp4-associated protein; Trpc4-associated protein; Protein TRUSS
Uniprot Description
Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. The DCX(TRUSS) complex specifically mediates the polyubiquitination and subsequent degradation of MYC. Also participates in the activation of NFKB1 in response to ligation of TNFRSF1A, possibly by linking TNFRSF1A to the IKK signalosome. Involved in JNK activation via its interaction with TRAF2. Also involved in elevation of endoplasmic reticulum Ca2+ storage reduction in response to CHRM1.
Research Articles on TRPC4AP
Similar Products
Product Notes
The TRPC4AP trpc4ap (Catalog #AAA9603719) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRPC4AP Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TRPC4AP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the TRPC4AP trpc4ap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRPC4AP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
