Mouse Mannose Associated Serine Protease 1 (MASP1) ELISA Kit | MASP1 elisa kit

Mouse Mannose Associated Serine Protease 1 (MASP1) ELISA Kit

Cat. Num. AAA2703864

• Gene Names: MASP1; 3MC1; MAP1; MASP; RaRF; CRARF; MASP3; MAp44; PRSS5; CRARF1
• Reactivity: Mouse
• Synonyms: Mannose Associated Serine Protease 1 (MASP1); Mouse Mannose Associated Serine Protease 1 (MASP1) ELISA Kit; CRARF; PRSS5; Mannan-Binding Lectin Serine Peptidase 1; Ra-reactive factor serine protease p100; Complement-activating component of Ra-reactive factor; Serine protease 5; MASP1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mannose Associated Serine Protease 1 (MASP1).; No significant cross-reactivity or interference between Mannose Associated Serine Protease 1 (MASP1) and analogues was observed.
• Sequence Length: 615

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 15.62-1000pg/mL
• Sensitivity: < 6.9pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mannose Associated Serine Protease 1 (MASP1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mannose Associated Serine Protease 1 (MASP1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MASP1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannose Associated Serine Protease 1 (MASP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mannose Associated Serine Protease 1 (MASP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannose Associated Serine Protease 1 (MASP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mannose Associated Serine Protease 1 (MASP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 21264359
• NCBI GeneID: 5648
• NCBI Accession #: NP_624302.1
• NCBI GenBank Nucleotide #: NM_139125.3
• UniProt Accession #: P48740; O95570; Q68D21; Q8IUV8; Q96RS4; Q9UF09; A8K542; A8K6M1; B4E2L7
• Molecular Weight: 68,918 Da
• NCBI Official Full Name: mannan-binding lectin serine protease 1 isoform 2
• NCBI Official Synonym Full Names: mannan binding lectin serine peptidase 1
• NCBI Official Symbol: MASP1
• NCBI Official Synonym Symbols: 3MC1; MAP1; MASP; RaRF; CRARF; MASP3; MAp44; PRSS5; CRARF1
• NCBI Protein Information: mannan-binding lectin serine protease 1
• UniProt Protein Name: Mannan-binding lectin serine protease 1
• Protein Family: Mannan-binding lectin serine protease
• UniProt Gene Name: MASP1
• UniProt Synonym Gene Names: CRARF; CRARF1; PRSS5; MASP-1; RaRF

NCBI Description

This gene encodes a serine protease that functions as a component of the lectin pathway of complement activation. The complement pathway plays an essential role in the innate and adaptive immune response. The encoded protein is synthesized as a zymogen and is activated when it complexes with the pathogen recognition molecules of lectin pathway, the mannose-binding lectin and the ficolins. This protein is not directly involved in complement activation but may play a role as an amplifier of complement activation by cleaving complement C2 or by activating another complement serine protease, MASP-2. The encoded protein is also able to cleave fibrinogen and factor XIII and may may be involved in coagulation. A splice variant of this gene which lacks the serine protease domain functions as an inhibitor of the complement pathway. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]

Uniprot Description

Functions in the lectin pathway of complement, which performs a key role in innate immunity by recognizing pathogens through patterns of sugar moieties and neutralizing them. The lectin pathway is triggered upon binding of mannan-binding lectin (MBL) and ficolins to sugar moieties which leads to activation of the associated proteases MASP1 and MASP2. Functions as an endopeptidase and may activate MASP2 or C2 or directly activate C3 the key component of complement reaction. Isoform 2 may have an inhibitory effect on the activation of the lectin pathway of complement or may cleave IGFBP5.