Western Blot (WB)
(TRPC4AP Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the TRPC4AP antibody detected the TRPC4AP protein (arrow).)
Rabbit anti-Human TRPC4AP Polyclonal Antibody | anti-TRPC4AP antibody
TRPC4AP, NT (TRPC4AP, C20orf188, TRRP4AP, Short transient receptor potential channel 4-associated protein, Protein TAP1, TNF-receptor ubiquitous scaffolding/signaling protein)
Gene Names
TRPC4AP; TRUSS; TRRP4AP; C20orf188
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
TRPC4AP; Polyclonal Antibody; NT (TRPC4AP; C20orf188; TRRP4AP; Short transient receptor potential channel 4-associated protein; Protein TAP1; TNF-receptor ubiquitous scaffolding/signaling protein); Anti -TRPC4AP; anti-TRPC4AP antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-TRPC4AP antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunogen
TRPC4AP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-75 amino acids from the N-terminal region of human TRPC4AP.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(TRPC4AP Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the TRPC4AP antibody detected the TRPC4AP protein (arrow).)
Western Blot (WB)
(TRPC4AP Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the TRPC4AP antibody detected the TRPC4AP protein (arrow).)
Related Product Information for anti-TRPC4AP antibody
Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. The DCX(TRUSS) complex specifically mediates the polyubiquitination and subsequent degradation of MYC. Also participates in the activation of NFKB1 in response to ligation of TNFRSF1A, possibly by linking TNFRSF1A to the IKK signalosome. Involved in JNK activation via its interaction with TRAF2. Also involved in elevation of endoplasmic reticulum Ca(2+) storage reduction in response to CHRM1.
Product Categories/Family for anti-TRPC4AP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
90,852 Da
NCBI Official Full Name
TRPC4AP protein, partial
NCBI Official Synonym Full Names
transient receptor potential cation channel, subfamily C, member 4 associated protein
NCBI Official Symbol
TRPC4AP
NCBI Official Synonym Symbols
TRUSS; TRRP4AP; C20orf188
NCBI Protein Information
short transient receptor potential channel 4-associated protein; TRP4-associated protein; trpc4-associated protein; TNF-receptor ubiquitous scaffolding/signaling protein; short transient receptor potential channel 4 associated protein; tumor necrosis factor receptor-associated ubiquitous scaffolding and signaling protein
UniProt Protein Name
Short transient receptor potential channel 4-associated protein
UniProt Gene Name
TRPC4AP
UniProt Synonym Gene Names
C20orf188; TRRP4AP; Trp4-associated protein; Trpc4-associated protein
UniProt Entry Name
TP4AP_HUMAN
Uniprot Description
TRPC4AP: Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. The DCX(TRUSS) complex specifically mediates the polyubiquitination and subsequent degradation of MYC. Also participates in the activation of NFKB1 in response to ligation of TNFRSF1A, possibly by linking TNFRSF1A to the IKK signalosome. Involved in JNK activation via its interaction with TRAF2. Also involved in elevation of endoplasmic reticulum Ca(2+) storage reduction in response to CHRM1. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 20q11.22
Cellular Component: plasma membrane
Molecular Function: protein binding; phosphatase binding
Biological Process: ubiquitin-dependent protein catabolic process; protein ubiquitination; transmembrane transport
Research Articles on TRPC4AP
Similar Products
Product Notes
The TRPC4AP trpc4ap (Catalog #AAA641529) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRPC4AP, NT (TRPC4AP, C20orf188, TRRP4AP, Short transient receptor potential channel 4-associated protein, Protein TAP1, TNF-receptor ubiquitous scaffolding/signaling protein) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRPC4AP can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the TRPC4AP trpc4ap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRPC4AP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
