Western Blot (WB)
(Western blot analysis of RAB11FIP5 using COS7 whole cell lysates)
Rabbit RAB11FIP5 Polyclonal Antibody | anti-RAB11FIP5 antibody
RAB11FIP5 Antibody
Gene Names
RAB11FIP5; GAF1; pp75; RIP11
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
RAB11FIP5; Polyclonal Antibody; RAB11FIP5 Antibody; GAF 1; Gaf-1; GAF1; gamma SNAP associated factor 1; Gamma-SNAP-associated factor 1; KIAA0857; Phosphoprotein pp75; pp75; RAB11 family interacting protein 5 (class I); Rab11 family-interacting protein 5; Rab11 FIP5; Rab11 interacting protein; Rab11 interacting protein Rip11; Rab11-FIP5; Rab11-interacting protein Rip11; Rab11fip5; RFIP5_HUMAN; RIP11; anti-RAB11FIP5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
RAB11FIP5 antibody detects endogenous levels of total RAB11FIP5
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
653
Applicable Applications for anti-RAB11FIP5 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human RAB11FIP5
Subcellular Location
Cytoplasm. Recycling Endosome Membrane. Mitochondrion Membrane.
Tissue Specificity
Detected at low levels in heart, brain, placenta, lung, liver, adipocytes, kidney, spleen, skeletal muscle and pancreas.
Predicted Cross Reactivity
Pig, Bovine, Horse, Dog
Similarity
Pig (100%), Bovine (100%), Horse (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of RAB11FIP5 using COS7 whole cell lysates)
Western Blot (WB)
(Western blot analysis of RAB11FIP5 using COS7 whole cell lysates)
Related Product Information for anti-RAB11FIP5 antibody
Function: Rab effector involved in protein trafficking from apical recycling endosomes to the apical plasma membrane. Involved in insulin granule exocytosis. May regulate V-ATPase intracellular transport in response to extracellular acidosis.
Subunit Structure: Forms a heterooligomeric complex with RAB11FIP4. Binds NAPG and TROVE2. Binds RAB11A that has been activated by GTP binding.
Post-translational Modifications: Phosphorylated on serine and threonine residues. Phosphorylation at Ser-357 is PKA-dependent.
Similarity: Binds to vesicles enriched in neutral phospholipids via its C2 domain. The interaction is favored by Mg2+ rather than Ca2+.
Subunit Structure: Forms a heterooligomeric complex with RAB11FIP4. Binds NAPG and TROVE2. Binds RAB11A that has been activated by GTP binding.
Post-translational Modifications: Phosphorylated on serine and threonine residues. Phosphorylation at Ser-357 is PKA-dependent.
Similarity: Binds to vesicles enriched in neutral phospholipids via its C2 domain. The interaction is favored by Mg2+ rather than Ca2+.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 70 kDa
Predicted: 71 kDa
Predicted: 71 kDa
NCBI Official Full Name
rab11 family-interacting protein 5
NCBI Official Synonym Full Names
RAB11 family interacting protein 5
NCBI Official Symbol
RAB11FIP5
NCBI Official Synonym Symbols
GAF1; pp75; RIP11
NCBI Protein Information
rab11 family-interacting protein 5
UniProt Protein Name
Rab11 family-interacting protein 5
Protein Family
UniProt Gene Name
RAB11FIP5
UniProt Synonym Gene Names
GAF1; KIAA0857; RIP11; Rab11-FIP5; Gaf-1
Uniprot Description
Rab effector involved in protein trafficking from apical recycling endosomes to the apical plasma membrane. Involved in insulin granule exocytosis. May regulate V-ATPase intracellular transport in response to extracellular acidosis.
Research Articles on RAB11FIP5
Similar Products
Product Notes
The RAB11FIP5 rab11fip5 (Catalog #AAA9610112) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RAB11FIP5 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RAB11FIP5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the RAB11FIP5 rab11fip5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAB11FIP5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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