Rabbit anti-Human, Mouse TRIM27 Polyclonal Antibody | anti-TRIM27 antibody

TRIM27 Antibody

Cat. Num. AAA9416336

• Gene Names: TRIM27; RFP; RNF76
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry
• Purity: Antigen Affinity Purification
• Synonyms: TRIM27; Polyclonal Antibody; TRIM27 Antibody; RFP; RNF76; anti-TRIM27 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total TRIM27 protein.
• Purity/Purification: Antigen Affinity Purification
• Form/Format: Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Concentration: 2.3 mg/ml (varies by lot)
• Sequence Length: 513

• Applicable Applications for anti-TRIM27 antibody: Immunohistochemistry (IHC)
• Application Notes: Immunohistochemistry: 1:25-1:100
• Immunogen: Synthetic peptide of human TRIM27
• Immunogen Type: Peptide
• Preparation and Storage: Store at -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using at dilution 1/35.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using at dilution 1/35.)

Related Product Information for anti-TRIM27 antibody

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells.Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein.

Product Categories/Family for anti-TRIM27 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 5730009
• NCBI GeneID: 5987
• NCBI Accession #: NP_006501.1
• NCBI GenBank Nucleotide #: NM_006510.4
• UniProt Accession #: P14373; Q5RJA8; Q5ST26; Q6LA73; Q6NXR9; Q9BZY6; Q9UJL3; A2BE15
• Molecular Weight: 41,439 Da
• NCBI Official Full Name: zinc finger protein RFP
• NCBI Official Synonym Full Names: tripartite motif containing 27
• NCBI Official Symbol: TRIM27
• NCBI Official Synonym Symbols: RFP; RNF76
• NCBI Protein Information: zinc finger protein RFP
• UniProt Protein Name: Zinc finger protein RFP
• Protein Family: Zinc finger protein
• UniProt Gene Name: TRIM27
• UniProt Synonym Gene Names: RFP; RNF76
• UniProt Entry Name: TRI27_HUMAN

Uniprot Description

TRIM27: Has a transcriptional repressor activity by cooperating with EPC1. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. May function in male germ cell development. Has DNA-binding activity and preferentially bound to double-stranded DNA. E3 ubiquitin-protein ligase that mediates ubiquitination of PIK3C2B and inhibits its activity; mediates the formation of 'Lys-48'-linked polyubiquitin chains; the function inhibits CD4 T-cell activation. Defects in TRIM27 are a cause of thyroid papillary carcinoma (TPC). TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. A chromosomal aberration involving TRIM27/RFP is found in thyroid papillary carcinomas. Translocation t(6;10)(p21.3;q11.2) with RET. The translocation generates TRIM27/RET and delta TRIM27/RET oncogenes. Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; Motility/polarity/chemotaxis; Oncoprotein; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 6p22

Cellular Component: cytoplasm; endosome; integral to plasma membrane; membrane; nuclear membrane; nucleoplasm; nucleus; retromer complex

Molecular Function: identical protein binding; metal ion binding; nucleic acid binding; protein binding; transmembrane receptor protein tyrosine kinase activity; ubiquitin-protein ligase activity

Biological Process: cell proliferation; innate immune response; negative regulation of adaptive immune response; negative regulation of gene expression, epigenetic; negative regulation of protein kinase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of tumor necrosis factor production; negative regulation of viral transcription; positive regulation of transcription factor activity; retrograde transport, endosome to Golgi; spermatogenesis