Western Blot (WB)
(Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-4: Hela, 293T, RAW264.7 and NIH/3T3 cell lysates, Primary antibody: 863757(EEA1 Antibody) at dilution 1/450, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)
Rabbit anti-Human, Mouse EEA1 Polyclonal Antibody | anti-EEA1 antibody
EEA1 Rabbit pAb
Gene Names
EEA1; MST105; ZFYVE2; MSTP105
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
Affinity purified
Synonyms
EEA1; Polyclonal Antibody; EEA1 Rabbit pAb; early endosome antigen 1; MST105; ZFYVE2; MSTP105; anti-EEA1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Applicable Applications for anti-EEA1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
WB: 1:200-1:1000
ELISA: 1:5000-1:10,000
ELISA: 1:5000-1:10,000
Immunogen
Synthetic peptide of human EEA1
Conjugation
Unconjugated
Molecular Function
Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.MiscellaneousAntibodies against EEA1 are found in sera from patients with subacute cutaneous lupus erythematosus and other autoimmune diseases.RegionsFeature keyPosition(s)Description
Modification
Unmodified
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-4: Hela, 293T, RAW264.7 and NIH/3T3 cell lysates, Primary antibody: 863757(EEA1 Antibody) at dilution 1/450, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)
Western Blot (WB)
(Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-4: Hela, 293T, RAW264.7 and NIH/3T3 cell lysates, Primary antibody: 863757(EEA1 Antibody) at dilution 1/450, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)
Related Product Information for anti-EEA1 antibody
Essential for early endosomal membrane fusion and trafficking. The carboxy terminus of EEA1 contains a FYVE domain which binds to phosphatidylinositol-3-phosphate (PtdIns(3)P), targeting EEA1 to early endosomes. The stable association of EEA1 with the endosomal membrane is regulated by PI3 kinase, Rab5 and calcium/calmodulin. Once on the membrane, EEA1 interacts with Rab5, NSF and syntaxin 13 to promote early endosomal membrane docking and fusion.
Product Categories/Family for anti-EEA1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
162,466 Da
NCBI Official Full Name
Homo sapiens early endosome antigen 1 (EEA1), mRNA
NCBI Official Synonym Full Names
early endosome antigen 1
NCBI Official Symbol
EEA1
NCBI Official Synonym Symbols
MST105; ZFYVE2; MSTP105
NCBI Protein Information
early endosome antigen 1; early endosome antigen 1, 162kD; early endosome-associated protein; endosome-associated protein p162; zinc finger FYVE domain-containing protein 2
UniProt Protein Name
Early endosome antigen 1
Protein Family
UniProt Gene Name
EEA1
UniProt Synonym Gene Names
ZFYVE2
UniProt Entry Name
EEA1_HUMAN
Research Articles on EEA1
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Product Notes
The EEA1 eea1 (Catalog #AAA4758720) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EEA1 Rabbit pAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EEA1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). WB: 1:200-1:1000 ELISA: 1:5000-1:10,000. Researchers should empirically determine the suitability of the EEA1 eea1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EEA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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