Rabbit TRIM27 Polyclonal Antibody | anti-TRIM27 antibody

TRIM27 antibody

Cat. Num. AAA9409150

• Gene Names: TRIM27; RFP; RNF76
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: TRIM27; Polyclonal Antibody; TRIM27 antibody; RFP; RNF76; anti-TRIM27 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total TRIM27 antibody.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 513

• Applicable Applications for anti-TRIM27 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human TRIM27.
• Target Name: TRIM27
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using TRIM27 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cell using TRIM27 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-TRIM27 antibody

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein.

Product Categories/Family for anti-TRIM27 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 5730009
• NCBI GeneID: 5987
• NCBI Accession #: NP_006501.1
• NCBI GenBank Nucleotide #: NM_006510.4
• UniProt Accession #: P14373; Q5RJA8; Q5ST26; Q6LA73; Q6NXR9; Q9BZY6; Q9UJL3; A2BE15
• Molecular Weight: 41,439 Da
• NCBI Official Full Name: zinc finger protein RFP
• NCBI Official Synonym Full Names: tripartite motif containing 27
• NCBI Official Symbol: TRIM27
• NCBI Official Synonym Symbols: RFP; RNF76
• NCBI Protein Information: zinc finger protein RFP
• UniProt Protein Name: Zinc finger protein RFP
• Protein Family: Zinc finger protein
• UniProt Gene Name: TRIM27
• UniProt Synonym Gene Names: RFP; RNF76
• UniProt Entry Name: TRI27_HUMAN

NCBI Description

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein. [provided by RefSeq, Jul 2008]

Uniprot Description

TRIM27: Has a transcriptional repressor activity by cooperating with EPC1. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. May function in male germ cell development. Has DNA-binding activity and preferentially bound to double-stranded DNA. E3 ubiquitin-protein ligase that mediates ubiquitination of PIK3C2B and inhibits its activity; mediates the formation of 'Lys-48'-linked polyubiquitin chains; the function inhibits CD4 T-cell activation. Defects in TRIM27 are a cause of thyroid papillary carcinoma (TPC). TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. A chromosomal aberration involving TRIM27/RFP is found in thyroid papillary carcinomas. Translocation t(6;10)(p21.3;q11.2) with RET. The translocation generates TRIM27/RET and delta TRIM27/RET oncogenes. Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Oncoprotein; Ubiquitin conjugating system; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 6p22

Cellular Component: nucleoplasm; PML body; nuclear membrane; membrane; integral to plasma membrane; retromer complex; early endosome; cytoplasm; nucleus; endosome

Molecular Function: protein binding; DNA binding; nucleic acid binding; zinc ion binding; metal ion binding; ubiquitin-protein ligase activity; transmembrane receptor protein tyrosine kinase activity; ligase activity

Biological Process: cell proliferation; negative regulation of viral transcription; peptidyl-tyrosine phosphorylation; transcription, DNA-dependent; negative regulation of gene expression, epigenetic; negative regulation of adaptive immune response; innate immune response; negative regulation of protein kinase activity; positive regulation of transcription factor activity; spermatogenesis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of tumor necrosis factor production; retrograde transport, endosome to Golgi