Western Blot (WB)
(TRIM27 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TRIM27 antibody detected the TRIM27 protein (arrow).)
Rabbit TRIM27 Polyclonal Antibody | anti-TRIM27 antibody
TRIM27 Antibody (C-term)
Western Blot (WB)
(TRIM27 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TRIM27 antibody detected the TRIM27 protein (arrow).)
Western Blot (WB)
(TRIM27 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TRIM27 antibody detected the TRIM27 protein (arrow).)
family. The TRIM motif includes three zinc-binding domains, a RING,
a B-box type 1 and a B-box type 2, and a coiled-coil region. This
protein localizes to the nuclear matrix. It interacts with the
enhancer of polycomb protein and represses gene transcription. It
is also thought to be involved in the differentiation of male germ
cells. Fusion of the N-terminus of this protein with the truncated
C-terminus of the RET gene product has been shown to result in
production of the ret transforming protein.
Barcellos, L.F., et al. PLoS Genet. 5 (10), E1000696 (2009) :
Tezel, G.G., et al. Pathol. Res. Pract. 205(6):403-408(2009)
Li, X., et al. Virology 360(2):419-433(2007)
Lim, J., et al. Cell 125(4):801-814(2006)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein. [provided by RefSeq, Jul 2008]
Uniprot Description
TRIM27: Has a transcriptional repressor activity by cooperating with EPC1. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. May function in male germ cell development. Has DNA-binding activity and preferentially bound to double-stranded DNA. E3 ubiquitin-protein ligase that mediates ubiquitination of PIK3C2B and inhibits its activity; mediates the formation of 'Lys-48'-linked polyubiquitin chains; the function inhibits CD4 T-cell activation. Defects in TRIM27 are a cause of thyroid papillary carcinoma (TPC). TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. A chromosomal aberration involving TRIM27/RFP is found in thyroid papillary carcinomas. Translocation t(6;10)(p21.3;q11.2) with RET. The translocation generates TRIM27/RET and delta TRIM27/RET oncogenes. Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; EC 6.3.2.-; Ubiquitin conjugating system; Oncoprotein
Chromosomal Location of Human Ortholog: 6p22
Cellular Component: nucleoplasm; PML body; nuclear membrane; membrane; retromer complex; integral to plasma membrane; early endosome; cytoplasm; nucleus; endosome
Molecular Function: protein binding; DNA binding; nucleic acid binding; zinc ion binding; metal ion binding; ubiquitin-protein ligase activity; transmembrane receptor protein tyrosine kinase activity; ligase activity
Biological Process: cell proliferation; negative regulation of viral transcription; peptidyl-tyrosine phosphorylation; transcription, DNA-dependent; negative regulation of gene expression, epigenetic; negative regulation of adaptive immune response; negative regulation of protein kinase activity; innate immune response; positive regulation of transcription factor activity; spermatogenesis; negative regulation of tumor necrosis factor production; negative regulation of transcription from RNA polymerase II promoter; retrograde transport, endosome to Golgi
Research Articles on TRIM27
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Product Notes
The TRIM27 trim27 (Catalog #AAA9210483) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 453-482. The TRIM27 Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's TRIM27 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the TRIM27 trim27 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIM27, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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