SDS-Page
PNMT recombinant protein
PNMT, 1-282aa, Human, E Coli
Gene Names
PNMT; PENT; PNMTase
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
PNMT; 1-282aa; Human; E Coli; Phenylethanolamine N-methyltransferase; PENT; PNMTase; Noradrenaline-N-methyltransferase; PNMT recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol
Concentration
1.0 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MSGADRSPNA GAAPDSAPGQ AAVASAYQRF EPRAYLRNNY APPRGDLCNP NGVGPWKLRC LAQTFATGEV SGRTLIDIGS GPTVYQLLSA CSHFEDITMT DFLEVNRQEL GRWLQEEPGA FNWSMYSQHA CLIEGKGECW QDKERQLRAR VKRVLPIDVH QPQPLGAGSP APLPADALVS AFCLEAVSPD LASFQRALDH ITTLLRPGGH LLLIGALEES WYLAGEARLT VVPVSEEEVR EALVRSGYKV RDLRTYIMPA HLQTGVDDVK GVFFAWAQKV GL
Sequence Length
282
Applicable Applications for PNMT recombinant protein
SDS-PAGE
Antigen Species
Human
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for PNMT recombinant protein
PNMT, also known as phenylethanolamine N-methyltransferase, is an enzyme found in the adrenal medulla that catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. Recombinant PNMT protein was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for PNMT recombinant protein
References
Kaneda N., et al. (1998) Biochem Biophys Res Commun. 249 (2):405-9.; Ji Y., et al. (2005) J. Neurochem. 95:1766-1776.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30.8 Da (282aa), confirmed by MALDI-TOF.
NCBI Official Full Name
phenylethanolamine N-methyltransferase
NCBI Official Synonym Full Names
phenylethanolamine N-methyltransferase
NCBI Official Symbol
PNMT
NCBI Official Synonym Symbols
PENT; PNMTase
NCBI Protein Information
phenylethanolamine N-methyltransferase
UniProt Protein Name
Phenylethanolamine N-methyltransferase
Protein Family
UniProt Gene Name
PNMT
UniProt Synonym Gene Names
PENT; PNMTase
NCBI Description
The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012]
Uniprot Description
Converts noradrenaline to adrenaline.
Research Articles on PNMT
Similar Products
Product Notes
The PNMT pnmt (Catalog #AAA203446) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PNMT can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the PNMT pnmt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MSGADRSPNA GAAPDSAPGQ AAVASAYQRF EPRAYLRNNY APPRGDLCNP NGVGPWKLRC LAQTFATGEV SGRTLIDIGS GPTVYQLLSA CSHFEDITMT DFLEVNRQEL GRWLQEEPGA FNWSMYSQHA CLIEGKGECW QDKERQLRAR VKRVLPIDVH QPQPLGAGSP APLPADALVS AFCLEAVSPD LASFQRALDH ITTLLRPGGH LLLIGALEES WYLAGEARLT VVPVSEEEVR EALVRSGYKV RDLRTYIMPA HLQTGVDDVK GVFFAWAQKV GL. It is sometimes possible for the material contained within the vial of "PNMT, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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