Rabbit anti-Human ARC Polyclonal Antibody | anti-ARC antibody

ARC (E194) Polyclonal Antibody

Cat. Num. AAA3000323

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ARC; Polyclonal Antibody; ARC (E194) Polyclonal Antibody; Nucleolar protein 3; Apoptosis repressor with CARD; Muscle-enriched cytoplasmic protein; Myp; Nucleolar protein of 30 kDa; Nop30; NOL3; NOP; anti-ARC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ARC (E194) polyclonal antibody detects endogenous levels of ARC protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 219

• Applicable Applications for anti-ARC antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 161-210 of Human ARC.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ARC (E194) pAb at 1:500 dilutionLane1:HCT116 whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (30ug) Lane3:L02 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ARC (E194) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Western Blot (WB)

(Western Blot (WB) analysis of ARC (E194) pAb at 1:500 dilutionLane1:H9C2 whole cell lysate (40ug) Lane2:SP2/0 whole cell lysate (40ug) Lane3:Hela whole cell lysate (40ug) Lane4:The Brain tissue lysate of Rat (30ug) Lane5:The Brain tissue lysate of Mouse (30ug))

Related Product Information for anti-ARC antibody

Growth factor stimulation has been shown to induce the expression of immediate early genes in non-neuronal cells, which encode a variety of molecules that are potentially involved in long-term cellular responses. Similar responses induced by neurotransmitter stimulation have also been seen in neuronal cells and evidence suggests that protein synthesis is required for long-term synaptic plasticity. Arc (for activity-regulated cytoskeleton-associated protein) is a growth factor and immediate early gene that is enriched in brain. Arc mRNA and protein levels are induced by neuronal activity, which is necessary to stimulate neuroplasticity, indicating a potential role for Arc in activitydependent changes in dendrite function. Arc expression has been detected in neuronal cell bodies and dendrites in the hippocampus, amygdala, hypothalamus, striatum and cortex. Arc has been shown to localize to the cytoskeleton of neuronal cells and appears to co-localize with F-Actin, although it may associate with an Actin-associated protein rather than directly with F-Actin. It has been shown that cocaine-stimulated neuronal activity results in increased Arc mRNA levels in striatum.

NCBI and Uniprot Product Information

• NCBI GI #: 297632352
• NCBI Accession #: NP_001171986.1
• NCBI GenBank Nucleotide #: NP_001171986.1
• UniProt Accession #: O60936
• Molecular Weight: ~ 27kDa
• NCBI Official Full Name: nucleolar protein 3 isoform Nop30
• UniProt Protein Name: Nucleolar protein 3
• Protein Family: Arcelin
• UniProt Gene Name: NOL3
• UniProt Synonym Gene Names: ARC; NOP; Myp; Nop30
• UniProt Entry Name: NOL3_HUMAN

Uniprot Description

Function: Isoform 1 may be involved in RNA splicing.Isoform 2 may inhibit apoptosis.

Subunit structure: Interacts with TFPT

By similarity. Isoform 1 oligomerizes and binds to SFRS9/SRp30C and also interacts with NPM1. Isoform 2 binds CASP2/caspase-2 and CASP8/caspase-8 and inhibits caspase-8 activity. Ref.1

Subcellular location: Isoform 1: Nucleus › nucleolus. Isoform 2: Cytoplasm.

Tissue specificity: Highly expressed in heart and skeletal muscle. Detected at low levels in placenta, liver, kidney and pancreas.

Sequence similarities: Contains 1 CARD domain.