Human TIMP-3 ELISA Kit | TIMP-3 elisa kit

TIMP-3 Human ELISA Kit

Cat. Num. AAA355240

• Gene Names: TIMP3; SFD; K222; K222TA2; HSMRK222
• Reactivity: Human
• Applications: ELISA
• Synonyms: TIMP-3; TIMP-3 Human ELISA Kit; Human TIMP-3; TIMP-3 elisa kit

• Reactivity: Human

• Applicable Applications for TIMP-3 elisa kit: Quantitative sELISA (EIA)
• Application Notes: For quantitative detection of TIMP-3 in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
• Samples: Human serum, plasma, body fluids, tissue lysates or cell culture supernatants
• Assay Type: Quantitative Sandwich
• Detection Range: 156 pg/ml-10,000 pg/ml
• Sensitivity: < 2 pg/ml
• Preparation and Storage: Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TIMP-3 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-TIMP-3 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-TIMP-3 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TIMP-3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TIMP-3 can be calculated.

Background/Introduction: Metalloproteinase inhibitor 3 is an protein that in humans is encoded by the TIMP3 gene, which maps to to chromosome 22. This gene belongs to the tissue inhibitor of metalloproteinases gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. The balance of MMP and TIMP determines the net migratory capacity of DCs. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy.

NCBI and Uniprot Product Information

• NCBI GI #: 998826
• NCBI GeneID: 7078
• UniProt Accession #: P35625; Q5THV4; Q9UC74; Q9UGS2; B2RBY9
• Molecular Weight: 24,145 Da
• NCBI Official Full Name: TIMP-3
• NCBI Official Synonym Full Names: TIMP metallopeptidase inhibitor 3
• NCBI Official Symbol: TIMP3
• NCBI Official Synonym Symbols: SFD; K222; K222TA2; HSMRK222
• NCBI Protein Information: metalloproteinase inhibitor 3; TIMP-3; MIG-5 protein; protein MIG-5; tissue inhibitor of metalloproteinases 3
• UniProt Protein Name: Metalloproteinase inhibitor 3
• UniProt Gene Name: TIMP3
• UniProt Synonym Gene Names: TIMP-3
• UniProt Entry Name: TIMP3_HUMAN

NCBI Description

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.

Subunit structure: Interacts with EFEMP1. Ref.16

Subcellular location: Secreted › extracellular space › extracellular matrix.

Involvement in disease: Sorsby fundus dystrophy (SFD) [MIM:136900]: Rare autosomal dominant macular disorder with an age of onset in the fourth decade. It is characterized by loss of central vision from subretinal neovascularization and atrophy of the ocular tissues. Generally, macular disciform degeneration develops in the patients eye within 6 months to 6 years.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.18 Ref.19 Ref.20 Ref.21 Ref.22

Sequence similarities: Belongs to the protease inhibitor I35 (TIMP) family. [View classification]Contains 1 NTR domain.