Rabbit TIMM9 Polyclonal Antibody | anti-TIMM9 antibody

TIMM9 Antibody

Cat. Num. AAA9603715

• Gene Names: TIMM9; TIM9; TIM9A
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: TIMM9; Polyclonal Antibody; TIMM9 Antibody; Mitochondrial import inner membrane translocase subunit Tim9; TIM9; TIM9_HUMAN; TIM9A; Timm9; TIMM9A; anti-TIMM9 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TIMM9 antibody detects endogenous levels of TIMM9.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 89

• Applicable Applications for anti-TIMM9 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: A synthesized peptide derived from human TIMM9.
• Subcellular Location: Mitochondrion Inner Membrane.
• Tissue Specificity: Ubiquitous, with highest expression in heart, kidney, liver and skeletal muscle.
• Predicted Cross Reactivity: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
• Similarity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunohistochemistry (IHC)

(MBS9603715 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Western Blot (WB)

(Western blot analysis of extracts of mouse liver tissue, using TIMM9 antibody.)

Related Product Information for anti-TIMM9 antibody

Function: Mitochondrial intermembrane chaperone that participates in the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. May also be required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.
Subunit Structure: Heterohexamer; composed of 3 copies of TIMM9 and 3 copies of TIMM10/TIM10A, named soluble 70 kDa complex. The complex forms a 6-bladed alpha-propeller structure and associates with the TIMM22 component of the TIM22 complex. Interacts with multi-pass transmembrane proteins in transit. Also forms a complex composed of TIMM9, TIMM10/TIM10A and FXC1/TIM10B.
Similarity: The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIMM9 from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane (Probable). Belongs to the small Tim family.

NCBI and Uniprot Product Information

• NCBI GI #: 751247012
• NCBI GeneID: 26520
• NCBI Accession #: NP_001291414.1
• NCBI GenBank Nucleotide #: NM_001304485.1
• UniProt Accession #: Q9Y5J7; B2R584
• Molecular Weight: Observed: 10 kDa; Predicted: 11 kDa
• NCBI Official Full Name: mitochondrial import inner membrane translocase subunit Tim9 isoform a
• NCBI Official Synonym Full Names: translocase of inner mitochondrial membrane 9
• NCBI Official Symbol: TIMM9
• NCBI Official Synonym Symbols: TIM9; TIM9A
• NCBI Protein Information: mitochondrial import inner membrane translocase subunit Tim9
• UniProt Protein Name: Mitochondrial import inner membrane translocase subunit Tim9
• Protein Family: Mitochondrial import inner membrane translocase
• UniProt Gene Name: TIMM9
• UniProt Synonym Gene Names: TIM9; TIM9A; TIMM9A

NCBI Description

TIMM9 belongs to a family of evolutionarily conserved proteins that are organized in heterooligomeric complexes in the mitochondrial intermembrane space. These proteins mediate the import and insertion of hydrophobic membrane proteins into the mitochondrial inner membrane.[supplied by OMIM, Apr 2004]

Uniprot Description

Mitochondrial intermembrane chaperone that participates in the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. May also be required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.