Rabbit TIMM13 Polyclonal Antibody | anti-TIMM13 antibody

TIMM13 Antibody

Cat. Num. AAA9611524

• Gene Names: TIMM13; ppv1; TIM13; TIM13B; TIMM13A; TIMM13B
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Zebrafish, Bovine, Horse, Chicken, Xenopus
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: TIMM13; Polyclonal Antibody; TIMM13 Antibody; Mitochondrial import inner membrane translocase subunit TIM13; mitochondrial import inner membrane translocase subunit Tim13B; ppv1; TIM 13; TIM13; TIM13_HUMAN; TIM13B; TIMM 13; Timm13; TIMM13A; TIMM13B; Translocase of inner mitochondrial membrane 13 homolog; anti-TIMM13 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Zebrafish, Bovine, Horse, Chicken, Xenopus
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TIMM13 Antibody detects endogenous levels of TIMM13.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 95

• Applicable Applications for anti-TIMM13 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human TIMM13.
• Tissue Specificity: Ubiquitous, with highest expression in heart, kidney, liver and skeletal muscle.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Subcellular Location: Mitochondrion
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from PC-3 cells, using TIMM13 Antibody. The lane on the left was treated with blocking peptide.)

Western Blot (WB)

(Western blot analysis of extracts from various samples, using TIMM13 Antibody. Lane 1: B16F10 treated with blocking peptide; Lane 2: B16F10; Lane 3: Rat brain; Lane 4: 293; Lane 5: Myeloma cells.)

Related Product Information for anti-TIMM13 antibody

Description: Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. The TIMM8-TIMM13 complex mediates the import of proteins such as TIMM23, SLC25A12/ARALAR1 and SLC25A13/ARALAR2, while the predominant TIMM9-TIMM10 70 kDa complex mediates the import of much more proteins.

Subunit Structure: Heterohexamer; composed of 3 copies of TIMM8 (TIMM8A or TIMM8B) and 3 copies of TIMM13, named soluble 70 kDa complex. Associates with the TIM22 complex, whose core is composed of TIMM22.

Similarity: The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIMM13 from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane (By similarity). Belongs to the small Tim family.

NCBI and Uniprot Product Information

• NCBI GI #: 11024700
• NCBI GeneID: 26517
• NCBI Accession #: NP_036590.1
• NCBI GenBank Nucleotide #: NP_036590.1
• UniProt Accession #: Q9Y5L4
• Molecular Weight: Observed: 11 kDa; Predicted: 11 kDa
• NCBI Official Full Name: mitochondrial import inner membrane translocase subunit Tim13
• NCBI Official Synonym Full Names: translocase of inner mitochondrial membrane 13
• NCBI Official Symbol: TIMM13
• NCBI Official Synonym Symbols: ppv1; TIM13; TIM13B; TIMM13A; TIMM13B
• NCBI Protein Information: mitochondrial import inner membrane translocase subunit Tim13
• UniProt Protein Name: Mitochondrial import inner membrane translocase subunit Tim13
• UniProt Gene Name: TIMM13
• UniProt Synonym Gene Names: TIM13B; TIMM13A; TIMM13B
• UniProt Entry Name: TIM13_HUMAN

NCBI Description

This gene encodes a member of the evolutionarily conserved TIMM (translocase of inner mitochondrial membrane) family of proteins that function as chaperones in the import of proteins from the cytoplasm into the mitochondrial inner membrane. Proteins of this family play a role in collecting substrate proteins from the translocase of the outer mitochondrial membrane (TOM) complex and delivering them to either the sorting and assembly machinery in the outer mitochondrial membrane (SAM) complex or the TIMM22 complex in the inner mitochondrial membrane. The encoded protein and the translocase of mitochondrial inner membrane 8a protein form a 70 kDa complex in the intermembrane space. [provided by RefSeq, Jul 2013]