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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using TGS1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

Rabbit anti-Human, Mouse TGS1 Polyclonal Antibody | anti-TGS1 antibody

TGS1 Polyclonal Antibody

Gene Names
TGS1; PIMT; PIPMT; NCOA6IP
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Synonyms
TGS1; Polyclonal Antibody; TGS1 Polyclonal Antibody; anti-TGS1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Sequence Length
853
Applicable Applications for anti-TGS1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB 1:500 - 1:2000
IHC 1:50 - 1:200
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human TGS1
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using TGS1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using TGS1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney using TGS1 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human kidney using TGS1 antibody at dilution of 1:100 (40x lens).)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
97kDa
NCBI Official Full Name
trimethylguanosine synthase
NCBI Official Synonym Full Names
trimethylguanosine synthase 1
NCBI Official Symbol
TGS1
NCBI Official Synonym Symbols
PIMT; PIPMT; NCOA6IP
NCBI Protein Information
trimethylguanosine synthase
UniProt Protein Name
Trimethylguanosine synthase
UniProt Gene Name
TGS1
UniProt Synonym Gene Names
HCA137; NCOA6IP; PIMT; PIMT; PIPMT
UniProt Entry Name
TGS1_HUMAN

Uniprot Description

PIMT: a nuclear protein and apparent methyltransferase. Binds S-adenosyl-L-methionine and RNA. Plays a role in transcriptional regulation. Interacts with NCOA, CBP, p300, and PPARBP. Ubiquitously expressed. High expression in heart, skeletal muscle, kidney, liver and placenta.

Protein type: RNA processing; Methyltransferase; EC 2.1.1.-; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 8q11

Cellular Component: nucleoplasm; small nuclear ribonucleoprotein complex; Cajal body; extracellular space; cytoplasm; nucleus; cytosol

Molecular Function: protein binding

Biological Process: spliceosomal snRNP biogenesis; regulation of transcription, DNA-dependent; transcription, DNA-dependent; ribonucleoprotein complex biogenesis and assembly; RNA capping; gene expression; cellular lipid metabolic process

Research Articles on TGS1

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Product Notes

The TGS1 tgs1 (Catalog #AAA9128601) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TGS1 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TGS1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB 1:500 - 1:2000 IHC 1:50 - 1:200. Researchers should empirically determine the suitability of the TGS1 tgs1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TGS1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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