Western Blot (WB)
(Western blot analysis of CDK5RAP1 Antibody (C-term) in K562 cell line lysates (35ug/lane). CDK5RAP1 (arrow) was detected using the purified Pab.)
Rabbit anti-Human CDK5RAP1 Polyclonal Antibody | anti-CDK5RAP1 antibody
CDK5RAP1, CT (CDK5RAP1, C20orf34, CDK5 regulatory subunit-associated protein 1, CDK5 activator-binding protein C42) (Azide free) (HRP)
Gene Names
CDK5RAP1; C42; CGI-05; HSPC167; C20orf34
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
CDK5RAP1; Polyclonal Antibody; CT (CDK5RAP1; C20orf34; CDK5 regulatory subunit-associated protein 1; CDK5 activator-binding protein C42) (Azide free) (HRP); CDK5 activator-binding protein C42; anti-CDK5RAP1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-CDK5RAP1 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
CDK5RAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 550~580 amino acids from the C-terminal region of human CDK5RAP1.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of CDK5RAP1 Antibody (C-term) in K562 cell line lysates (35ug/lane). CDK5RAP1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of CDK5RAP1 Antibody (C-term) in K562 cell line lysates (35ug/lane). CDK5RAP1 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with CDK5RAP1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with CDK5RAP1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(CDK5RAP1 Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(CDK5RAP1 Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-CDK5RAP1 antibody
References
Ching Y.-P., et.al., J. Biol. Chem. 277:15237-15240(2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
67,689 Da
NCBI Official Full Name
CDK5 regulatory subunit-associated protein 1 isoform d
NCBI Official Synonym Full Names
CDK5 regulatory subunit associated protein 1
NCBI Official Symbol
CDK5RAP1
NCBI Official Synonym Symbols
C42; CGI-05; HSPC167; C20orf34
NCBI Protein Information
CDK5 regulatory subunit-associated protein 1; CDK5 activator-binding protein C42
UniProt Protein Name
CDK5 regulatory subunit-associated protein 1
Protein Family
UniProt Gene Name
CDK5RAP1
UniProt Synonym Gene Names
C20orf34; CGI-05; HSPC167
UniProt Entry Name
CK5P1_HUMAN
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Product Notes
The CDK5RAP1 cdk5rap1 (Catalog #AAA6284450) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDK5RAP1, CT (CDK5RAP1, C20orf34, CDK5 regulatory subunit-associated protein 1, CDK5 activator-binding protein C42) (Azide free) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CDK5RAP1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-100 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CDK5RAP1 cdk5rap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDK5RAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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