Western Blot (WB)
(Western blotAll lanes: TGS1 antibody at 8ug/ml+ Mouse heart tissueGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 97 kDaObserved band size: 85 kDa)
Rabbit anti-Human Trimethylguanosine synthase Polyclonal Antibody | anti-TGS1 antibody
Rabbit anti-human Trimethylguanosine synthase polyclonal Antibody(TGS1)
Gene Names
TGS1; PIMT; PIPMT; NCOA6IP
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Synonyms
Trimethylguanosine synthase; Polyclonal Antibody; Rabbit anti-human Trimethylguanosine synthase polyclonal Antibody(TGS1); CLL-associated antigen KW-2; Cap-specific guanine-N2 methyltransferase; Hepatocellular carcinoma-associated antigen 137; Nuclear receptor coactivator 6-interacting protein; PRIP-interacting protein with methyltransferase motif; PIMT; PIPMT; HCA137; NCOA6IP; TGS1; anti-TGS1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation purified
Form/Format
Liquid
Sequence Length
853
Applicable Applications for anti-TGS1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Recommended dilution: WB:1:500-2000, IHC:1:20-1:200
Immunogen
Recombinant human Trimethylguanosine synthase protein(713-853AA)
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-23113 / sc-23114
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.
Western Blot (WB)
(Western blotAll lanes: TGS1 antibody at 8ug/ml+ Mouse heart tissueGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 97 kDaObserved band size: 85 kDa)
Western Blot (WB)
(Western blotAll lanes: TGS1 antibody at 8ug/ml+ Mouse heart tissueGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 97 kDaObserved band size: 85 kDa)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human kidney using MBS7000702 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human kidney using MBS7000702 at dilution 1:100)
Related Product Information for anti-TGS1 antibody
Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m7G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs). Plays a role in transcriptional regulation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
96,620 Da
NCBI Official Full Name
trimethylguanosine synthase isoform 1
NCBI Official Synonym Full Names
trimethylguanosine synthase 1
NCBI Official Symbol
TGS1
NCBI Official Synonym Symbols
PIMT; PIPMT; NCOA6IP
NCBI Protein Information
trimethylguanosine synthase
UniProt Protein Name
Trimethylguanosine synthase
Protein Family
UniProt Gene Name
TGS1
UniProt Synonym Gene Names
HCA137; NCOA6IP; PIMT; PIMT; PIPMT
UniProt Entry Name
TGS1_HUMAN
Uniprot Description
PIMT: a nuclear protein and apparent methyltransferase. Binds S-adenosyl-L-methionine and RNA. Plays a role in transcriptional regulation. Interacts with NCOA, CBP, p300, and PPARBP. Ubiquitously expressed. High expression in heart, skeletal muscle, kidney, liver and placenta.
Protein type: Methyltransferase; RNA processing; Nuclear receptor co-regulator; EC 2.1.1.-
Chromosomal Location of Human Ortholog: 8q11
Cellular Component: Cajal body; cytoplasm; cytosol; extracellular space; nucleolus; nucleoplasm; nucleus; small nuclear ribonucleoprotein complex
Molecular Function: protein binding
Biological Process: cellular lipid metabolic process; gene expression; regulation of transcription, DNA-dependent; ribonucleoprotein complex biogenesis and assembly; RNA capping; spliceosomal snRNP biogenesis; transcription, DNA-dependent
Research Articles on TGS1
Similar Products
Product Notes
The TGS1 tgs1 (Catalog #AAA7000702) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Trimethylguanosine synthase polyclonal Antibody(TGS1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Trimethylguanosine synthase can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Recommended dilution: WB:1:500-2000, IHC:1:20-1:200. Researchers should empirically determine the suitability of the TGS1 tgs1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Trimethylguanosine synthase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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