Western Blot (WB)
(Western blot analysis of extracts from HepG2 cells, using TEX14 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit anti-Human TEX14 Polyclonal Antibody | anti-TEX14 antibody
TEX14 Antibody
Gene Names
TEX14; CT113; SPGF23
Reactivity
Human
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
TEX14; Polyclonal Antibody; TEX14 Antibody; Cancer/testis antigen 113; CT113; Inactive serine/threonine protein kinase TEX14; Protein kinase like protein SgK307; Sugen kinase 307; Testis expressed Protein 14; Testis expressed sequence 14; Testis expressed sequence 14 protein; anti-TEX14 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
TEX14 Antibody detects endogenous levels of total TEX14.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
1451
Applicable Applications for anti-TEX14 antibody
ELISA (EIA)
Application Notes
WB: 1:500-1:2000
ELISA(peptide): 1:20,000-1:40,000
ELISA(peptide): 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human TEX14, corresponding to a region within the internal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from HepG2 cells, using TEX14 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from HepG2 cells, using TEX14 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-TEX14 antibody
Function: Required both for the formation of intercellular bridges during meiosis and for kinetochore-microtubule attachment during mitosis. Intercellular bridges are evolutionarily conserved structures that connect differentiating germ cells and are required for spermatogenesis and male fertility. Acts by promoting the conversion of midbodies into intercellular bridges via its interaction with CEP55: interaction with CEP55 inhibits the interaction between CEP55 and PDCD6IP/ALIX and TSG101, blocking cell abscission and leading to transform midbodies into intercellular bridges. Also plays a role during mitosis: recruited to kinetochores by PLK1 during early mitosis and regulates the maturation of the outer kinetochores and microtubule attachment. Has no protein kinase activity in vitro (By similarity).
Post Translational Modifications: Phosphorylated on Thr residues by CDK1 during early phases of mitosis, promoting the interaction with PLK1 and recruitment to kinetochores. Phosphorylated on Ser-437 by PLK1 during late prometaphase promotes the rapid depletion from kinetochores and its subsequent degradation by the APC/C complex.
Subcellular Location: Cytoplasm. Midbody. Chromosome>Centromere>Kinetochore. Note: Detected in the intercellular bridges that connect male germ cell daughter cells after cell division.
Tissue Specificity: Expression restricted to testis.
Subunit Structure: Interacts with KIF23 and RBM44. Interacts with CEP55; inhibiting interaction between CEP55 and PDCD6IP/ALIX and TSG101 (By similarity).
Similarity: The protein kinase domain is predicted to be catalytically inactive.The GPPX3Y motif mediates interaction with CEP55.Belongs to the protein kinase superfamily.
Post Translational Modifications: Phosphorylated on Thr residues by CDK1 during early phases of mitosis, promoting the interaction with PLK1 and recruitment to kinetochores. Phosphorylated on Ser-437 by PLK1 during late prometaphase promotes the rapid depletion from kinetochores and its subsequent degradation by the APC/C complex.
Subcellular Location: Cytoplasm. Midbody. Chromosome>Centromere>Kinetochore. Note: Detected in the intercellular bridges that connect male germ cell daughter cells after cell division.
Tissue Specificity: Expression restricted to testis.
Subunit Structure: Interacts with KIF23 and RBM44. Interacts with CEP55; inhibiting interaction between CEP55 and PDCD6IP/ALIX and TSG101 (By similarity).
Similarity: The protein kinase domain is predicted to be catalytically inactive.The GPPX3Y motif mediates interaction with CEP55.Belongs to the protein kinase superfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)167kD.
Predicted Molecular Weight: (Calculated)168kDa.
Predicted Molecular Weight: (Calculated)168kDa.
NCBI Official Full Name
inactive serine/threonine-protein kinase TEX14 isoform b
NCBI Official Synonym Full Names
testis expressed 14, intercellular bridge forming factor
NCBI Official Symbol
TEX14
NCBI Official Synonym Symbols
CT113; SPGF23
NCBI Protein Information
inactive serine/threonine-protein kinase TEX14
UniProt Protein Name
Inactive serine/threonine-protein kinase TEX14
Protein Family
UniProt Gene Name
TEX14
UniProt Synonym Gene Names
SGK307
UniProt Entry Name
TEX14_HUMAN
NCBI Description
The protein encoded by this gene is necessary for intercellular bridges in germ cells, which are required for spermatogenesis. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2011]
Research Articles on TEX14
Similar Products
Product Notes
The TEX14 tex14 (Catalog #AAA9612482) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TEX14 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TEX14 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). WB: 1:500-1:2000 ELISA(peptide): 1:20,000-1:40,000. Researchers should empirically determine the suitability of the TEX14 tex14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TEX14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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