Western Blot (WB)
(Western blot analysis of extracts from Huvec, using Mael Antibody. The lane on the left was treated with blocking peptide.)
Rabbit Mael Polyclonal Antibody | anti-MAEL antibody
Mael Antibody
Predicted Reactivity: Pig(88%), Horse(88%), Rabbit(100%), Dog(88%)
Predicted Reactivity: Pig(88%), Horse(88%), Rabbit(100%), Dog(88%)
ELISA(peptide): 1:20,000-1:40,000
Western Blot (WB)
(Western blot analysis of extracts from Huvec, using Mael Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Huvec, using Mael Antibody. The lane on the left was treated with blocking peptide.)
Subcellular Location: Cytoplasm. Nucleus. Note: Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. Specifically localizes to piP-bodies, a subset of the nuage which contains secondary piRNAs (By similarity).
Tissue Specificity: Testis-specific. Expressed in various cancer cell lines, probably due to demethylation of its promoter.
Subunit Structure: Interacts with SMARCB1, SIN3B and DDX4. Interacts with piRNA-associated proteins TDRD1, PIWIL1 and PIWIL2 (By similarity). Interacts with TEX19 (By similarity).
Similarity: Belongs to the maelstrom family.
NCBI and Uniprot Product Information
Predicted Molecular Weight: (Calculated)49kDa.
Uniprot Description
MAEL: Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Its association with piP-bodies suggests a participation in the secondary piRNAs metabolic process. Required for localization of germ-cell factors to the meiotic nuage. Belongs to the maelstrom family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Cancer Testis Antigen (CTA)
Chromosomal Location of Human Ortholog: 1q24.1
Cellular Component: XY body; autosome; perinuclear region of cytoplasm; cytoplasm; nucleus; chromatin
Molecular Function: DNA binding
Biological Process: fertilization; DNA damage response, signal transduction resulting in induction of apoptosis; DNA methylation during gametogenesis; multicellular organismal development; synapsis; cell morphogenesis; gene expression; regulation of organ growth; RNA-mediated gene silencing; spermatogenesis; negative regulation of transcription from RNA polymerase II promoter; male meiosis; cell differentiation; negative regulation of apoptosis
Research Articles on MAEL
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Product Notes
The MAEL mael (Catalog #AAA9612446) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Mael Antibody reacts with Human Predicted Reactivity: Pig(88%), Horse(88%), Rabbit(100%), Dog(88%) and may cross-react with other species as described in the data sheet. AAA Biotech's Mael can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). WB: 1:500-1:2000 ELISA(peptide): 1:20,000-1:40,000. Researchers should empirically determine the suitability of the MAEL mael for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mael, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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