Western Blot (WB)
(Western blot analysis of extracts from Hela cells, using Ninein - Centrosome Marker Antibody. The lane on the left was treated with blocking peptide.)
Rabbit anti-Human Ninein - Centrosome Marker Polyclonal Antibody | anti-NIN antibody
Ninein - Centrosome Marker Antibody
Gene Names
NIN; SCKL7
Reactivity
Human
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
Ninein - Centrosome Marker; Polyclonal Antibody; Ninein - Centrosome Marker Antibody; Centrosome Marker; Glycogen synthase kinase 3 beta interacting protein; GSK3B interacting protein; h Glycogen synthase kinase 3 beta-interacting protein; hNinein; KIAA1565; NIN; NIN protein; NIN_HUMAN; ninein (GSK3B interacting protein); Ninein; Ninein centrosomal protein; SCKL7; anti-NIN antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Ninein - Centrosome Marker Antibody detects endogenous levels of total Ninein - Centrosome Marker.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
1275
Applicable Applications for anti-NIN antibody
ELISA (EIA)
Application Notes
WB: 1:500-1:2000
ELISA(peptide): 1:20,000-1:40,000
ELISA(peptide): 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human Ninein - Centrosome Marker, corresponding to a region within C-terminal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from Hela cells, using Ninein - Centrosome Marker Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Hela cells, using Ninein - Centrosome Marker Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-NIN antibody
Function: Centrosomal protein required in the positioning and anchorage of the microtubule minus-end in epithelial cells (PubMed:15190203, PubMed:23386061). May also act as a centrosome maturation factor (PubMed:11956314). May play a role in microtubule nucleation, by recruiting the gamma-tubulin ring complex to the centrosome (PubMed:15190203). Overexpression does not perturb nucleation or elongation of microtubules but suppresses release of microtubules (PubMed:15190203). Required for centriole organization and microtubule anchoring at the mother centriole (PubMed:23386061).
Post Translational Modifications: Phosphorylated by AURKA/Aurora kinase A and PKA kinases but not CK2 or AURKB/ Aurora kinase B.
Subcellular Location: Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole. Note: Component of the core centrosome. Arranged in a tubular conformation with an open and a closed end within the centrosome. In the mother centrosome, it localizes at both ends of the centrosome tube, including the site of centrosome duplication, while in the daughter centrosome it is present only at the closed end. Requires PCM1 for centrosome localization. Localizes to the subdistal appendage region of the centriole in a DCTN1-dependent manner.Cytoplasm. Note: Seems to have a dominant-negative effect on localization of other isoforms, promoting their dissociation from the centrosome.
Tissue Specificity: Ubiquitous. Highly expressed in heart and skeletal muscle. Isoform 1 is more expressed than isoform 5.
Subunit Structure: Homooligomer. Interacts with GSK3B/GSK3-beta via its C-terminal domain (PubMed:11004522). Interacts with C14ORF166, such interaction may prevent its phosphorylation by GSK3B (PubMed:15147888). Interacts with AUNIP (via N-terminus) (PubMed:20596670). Identified in a complex with AUNIP and AURKA (PubMed:20596670). Interacts with CCDC120 (PubMed:28422092). Interacts (via C-terminus) with CEP250 (By similarity). Interacts with CEP170 (By similarity). Interacts with the gamma-tubulin ring complex component TUBGCP3 (By similarity). Interacts with gamma-tubulin (By similarity). Isoform 6 does not interact with CEP170 or CEP250 (By similarity).
Similarity: There is conflicting information regarding the regions required for centrosomal localization. One study shows that the region 1601-1682 is necessary and sufficient for targeting to the centrosome (PubMed:12927815). Another study shows that a separate region, 1291-1575, is important for centrosomal localization (PubMed:15190203). However, a third study shows that the coiled-coil region (373-1885) is not sufficient for centrosomal localization and instead localizes to cytoplasmic speckles (By similarity). The observed differences might be due to oligomerization of the longer coiled-coil domain-containing sequence, which would mask the shorter centrosomal targeting sequences (By similarity).The N-terminal domain is important for targeting to the mother centriole, although it is not sufficient by itself for centrosomal localization.
Post Translational Modifications: Phosphorylated by AURKA/Aurora kinase A and PKA kinases but not CK2 or AURKB/ Aurora kinase B.
Subcellular Location: Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole. Note: Component of the core centrosome. Arranged in a tubular conformation with an open and a closed end within the centrosome. In the mother centrosome, it localizes at both ends of the centrosome tube, including the site of centrosome duplication, while in the daughter centrosome it is present only at the closed end. Requires PCM1 for centrosome localization. Localizes to the subdistal appendage region of the centriole in a DCTN1-dependent manner.Cytoplasm. Note: Seems to have a dominant-negative effect on localization of other isoforms, promoting their dissociation from the centrosome.
Tissue Specificity: Ubiquitous. Highly expressed in heart and skeletal muscle. Isoform 1 is more expressed than isoform 5.
Subunit Structure: Homooligomer. Interacts with GSK3B/GSK3-beta via its C-terminal domain (PubMed:11004522). Interacts with C14ORF166, such interaction may prevent its phosphorylation by GSK3B (PubMed:15147888). Interacts with AUNIP (via N-terminus) (PubMed:20596670). Identified in a complex with AUNIP and AURKA (PubMed:20596670). Interacts with CCDC120 (PubMed:28422092). Interacts (via C-terminus) with CEP250 (By similarity). Interacts with CEP170 (By similarity). Interacts with the gamma-tubulin ring complex component TUBGCP3 (By similarity). Interacts with gamma-tubulin (By similarity). Isoform 6 does not interact with CEP170 or CEP250 (By similarity).
Similarity: There is conflicting information regarding the regions required for centrosomal localization. One study shows that the region 1601-1682 is necessary and sufficient for targeting to the centrosome (PubMed:12927815). Another study shows that a separate region, 1291-1575, is important for centrosomal localization (PubMed:15190203). However, a third study shows that the coiled-coil region (373-1885) is not sufficient for centrosomal localization and instead localizes to cytoplasmic speckles (By similarity). The observed differences might be due to oligomerization of the longer coiled-coil domain-containing sequence, which would mask the shorter centrosomal targeting sequences (By similarity).The N-terminal domain is important for targeting to the mother centriole, although it is not sufficient by itself for centrosomal localization.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)243kD.
Predicted Molecular Weight: (Calculated)243kDa.
Predicted Molecular Weight: (Calculated)243kDa.
NCBI Official Full Name
ninein (GSK3B interacting protein), isoform CRA_j
NCBI Official Synonym Full Names
ninein
NCBI Official Symbol
NIN
NCBI Official Synonym Symbols
SCKL7
NCBI Protein Information
ninein
UniProt Protein Name
Ninein
Protein Family
UniProt Gene Name
NIN
UniProt Synonym Gene Names
KIAA1565; hNinein; GSK3B-interacting protein
NCBI Description
This gene encodes one of the proteins important for centrosomal function. This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells. Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported. [provided by RefSeq, Jul 2008]
Uniprot Description
Centrosomal protein required in the positioning and anchorage of the microtubule minus-end in epithelial cells (PubMed:15190203, PubMed:23386061). May also act as a centrosome maturation factor (PubMed:11956314). May play a role in microtubule nucleation, by recruiting the gamma-tubulin ring complex to the centrosome (PubMed:15190203). Overexpression does not perturb nucleation or elongation of microtubules but suppresses release of microtubules (PubMed:15190203). Required for centriole organization and microtubule anchoring at the mother centriole (PubMed:23386061).
Research Articles on NIN
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Product Notes
The NIN nin (Catalog #AAA9612015) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ninein - Centrosome Marker Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Ninein - Centrosome Marker can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). WB: 1:500-1:2000 ELISA(peptide): 1:20,000-1:40,000. Researchers should empirically determine the suitability of the NIN nin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ninein - Centrosome Marker, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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