Western Blot (WB)
(Western blot analysis of extracts from K562, using SPC25 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit SPC25 Polyclonal Antibody | anti-SPC25 antibody
SPC25 Antibody
Gene Names
SPC25; AD024; SPBC25; hSpc25
Reactivity
HumanPredicted Reactivity: Bovine(88%), Horse(88%), Rabbit(88%), Dog(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
SPC25; Polyclonal Antibody; SPC25 Antibody; AD024; hSpc25; Kinetochore protein Spc25; SPBC25; SPC25_HUMAN; spindle pole body component 25 homolog; anti-SPC25 antibody
Host
Rabbit
Reactivity
Human
Predicted Reactivity: Bovine(88%), Horse(88%), Rabbit(88%), Dog(88%)
Predicted Reactivity: Bovine(88%), Horse(88%), Rabbit(88%), Dog(88%)
Clonality
Polyclonal
Isotype
IgG
Specificity
SPC25 Antibody detects endogenous levels of total SPC25.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
224
Applicable Applications for anti-SPC25 antibody
ELISA (EIA)
Application Notes
WB: 1:500-1:2000
ELISA(peptide): 1:20,000-1:40,000
ELISA(peptide): 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human SPC25, corresponding to a region within N-terminal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from K562, using SPC25 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from K562, using SPC25 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-SPC25 antibody
Function: Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:14699129, PubMed:14738735). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:14738735, PubMed:14699129). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020).
Subcellular Location: Nucleus. Chromosome>Centromere>Kinetochore. Note: Localizes to kinetochores from late prophase to anaphase (PubMed:14738735). Localizes specifically to the outer plate of the kinetochore (PubMed:14738735, PubMed:14699129).
Subunit Structure: Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C-terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end.
Similarity: Belongs to the SPC25 family.
Subcellular Location: Nucleus. Chromosome>Centromere>Kinetochore. Note: Localizes to kinetochores from late prophase to anaphase (PubMed:14738735). Localizes specifically to the outer plate of the kinetochore (PubMed:14738735, PubMed:14699129).
Subunit Structure: Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C-terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end.
Similarity: Belongs to the SPC25 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)26kD.
Predicted Molecular Weight: (Calculated)26kDa.
Predicted Molecular Weight: (Calculated)26kDa.
NCBI Official Full Name
kinetochore protein Spc25
NCBI Official Synonym Full Names
SPC25 component of NDC80 kinetochore complex
NCBI Official Symbol
SPC25
NCBI Official Synonym Symbols
AD024; SPBC25; hSpc25
NCBI Protein Information
kinetochore protein Spc25
UniProt Protein Name
Kinetochore protein Spc25
Protein Family
UniProt Gene Name
SPC25
UniProt Synonym Gene Names
SPBC25; hSpc25
UniProt Entry Name
SPC25_HUMAN
NCBI Description
This gene encodes a protein that may be involved in kinetochore-microtubule interaction and spindle checkpoint activity. [provided by RefSeq, Jul 2008]
Research Articles on SPC25
Similar Products
Product Notes
The SPC25 spc25 (Catalog #AAA9612506) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPC25 Antibody reacts with Human Predicted Reactivity: Bovine(88%), Horse(88%), Rabbit(88%), Dog(88%) and may cross-react with other species as described in the data sheet. AAA Biotech's SPC25 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). WB: 1:500-1:2000 ELISA(peptide): 1:20,000-1:40,000. Researchers should empirically determine the suitability of the SPC25 spc25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPC25, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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