Rabbit COMP Polyclonal Antibody | anti-COMP antibody

COMP Antibody

Cat. Num. AAA9612197

• Gene Names: COMP; MED; EDM1; EPD1; TSP5; PSACH; THBS5
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: COMP; Polyclonal Antibody; COMP Antibody; cartilage oligomeric matrix protein (pseudoachondroplasia; epiphyseal dysplasia 1; multiple); Cartilage oligomeric matrix protein; Cartilage oligomeric matrix protein precursor; COMP_HUMAN; EDM 1; EDM1; EPD 1; EPD1; Epiphyseal dysplasia 1; Epiphyseal dysplasia 1 multiple; Epiphyseal dysplasia multiple 1; MED; MGC13181; MGC149768; PSACH; pseudoachondroplasia (epiphyseal dysplasia 1; Pseudoachondroplasia; THBS 5; THBS5; Thrombospondin 5; Thrombospondin-5; Thrombospondin5; TSP5; anti-COMP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: COMP Antibody detects endogenous levels of total COMP.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 757

• Applicable Applications for anti-COMP antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human COMP, corresponding to a region within the internal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Human skeletal muscle, using COMP Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-COMP antibody

Function: May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7 (By similarity).

Subcellular Location: Secreted>Extracellular space>Extracellular matrix.

Tissue Specificity: Abundantly expressed in the chondrocyte extracellular matrix, and is also found in bone, tendon, ligament and synovium and blood vessels. Increased amounts are produced during late stages of osteoarthritis in the area adjacent to the main defect.

Subunit Structure: Pentamer; disulfide-linked. Exists in a more compact conformation in the presence of calcium and shows a more extended conformation in the absence of calcium. Interacts with ITGB3, ITGA5 and FN1. Binding to FN1 requires the presence of divalent cations (Ca(2+), Mg(2+) or Mn(2+)). The greatest amount of binding is seen in the presence of Mn(2+). Interacts with MATN1, MATN3, MATN4 and ACAN. Binds heparin, heparan sulfate and chondroitin sulfate. EDTA dimishes significantly its binding to ACAN and abolishes its binding to MATN3, MATN4 and chondroitin sulfate. Interacts with collagen I, II and IX, and interaction with these collagens is dependent on the presence of zinc ions. Interacts with ADAMTS12. Interacts with ITGA7 (By similarity).

Similarity: The cell attachment motif mediates the attachment to chondrocytes. It mediates the induction of both the IAP family of survival proteins and the antiapoptotic response.The TSP C-terminal domain mediates interaction with FN1 and ACAN.Each of the eight TSP type-3 repeats binds two calcium ions. The TSP C-terminal domain binds three calcium ions.Belongs to the thrombospondin family.

NCBI and Uniprot Product Information

• NCBI GI #: 40217843
• NCBI GeneID: 1311
• NCBI Accession #: NP_000086.2
• NCBI GenBank Nucleotide #: NP_000086.2
• UniProt Accession #: P49747
• Molecular Weight: Observed Molecular Weight: (Observed)82kD.; Predicted Molecular Weight: (Calculated)83kDa.
• NCBI Official Full Name: cartilage oligomeric matrix protein
• NCBI Official Synonym Full Names: cartilage oligomeric matrix protein
• NCBI Official Symbol: COMP
• NCBI Official Synonym Symbols: MED; EDM1; EPD1; TSP5; PSACH; THBS5
• NCBI Protein Information: cartilage oligomeric matrix protein
• UniProt Protein Name: Cartilage oligomeric matrix protein
• Protein Family: Complexin
• UniProt Gene Name: COMP
• UniProt Synonym Gene Names: COMP; TSP5
• UniProt Entry Name: COMP_HUMAN

NCBI Description

The protein encoded by this gene is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF-like and calcium-binding (thrombospondin-like) domains. Oligomerization results from formation of a five-stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Contraction or expansion of a 5 aa aspartate repeat and other mutations can cause pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED). [provided by RefSeq, Jul 2016]

Uniprot Description

COMP: May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7. Defects in COMP are the cause of multiple epiphyseal dysplasia type 1 (EDM1). EDM is a generalized skeletal dysplasia associated with significant morbidity. Joint pain, joint deformity, waddling gait, and short stature are the main clinical signs and symptoms. EDM is broadly categorized into the more severe Fairbank and the milder Ribbing types. Defects in COMP are the cause of pseudoachondroplasia (PSACH). PSAC is a dominantly inherited chondrodysplasia characterized by short stature and early-onset osteoarthrosis. PSACH is more severe than EDM1 and is recognized in early childhood. Belongs to the thrombospondin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: heparin binding; collagen binding; heparan sulfate proteoglycan binding; protein binding; protease binding; extracellular matrix structural constituent; calcium ion binding

Biological Process: limb development; extracellular matrix organization and biogenesis; organ morphogenesis; apoptosis; cell adhesion; skeletal development; negative regulation of apoptosis

Disease: Epiphyseal Dysplasia, Multiple, 1; Pseudoachondroplasia