Rabbit anti-Human, Mouse TAJ Polyclonal Antibody | anti-TNFRSF19 antibody

TAJ (TROY)

Cat. Num. AAA615872

• Gene Names: TNFRSF19; TAJ; TROY; TRADE; TAJ-alpha
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified; Purified by Protein G affinity chromatography.
• Synonyms: TAJ; Polyclonal Antibody; TAJ (TROY); Anti -TAJ (TROY); anti-TNFRSF19 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TAJ/TROY. Species Crossreactivity: mouse
• Purity/Purification: Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-TNFRSF19 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 0.5-2ug/ml (MW: 46kD).
• Immunogen: Synthetic peptide mixture corresponding to amino acids 29-44, 204-219, and 409-421 of human TROY (Genbank Accession No. AB040434).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of TAJ/TROY using MBS615872 in T98G, a human glioblastoma cell line lysate. A protein band of approximately 46 kD is detected.)

Related Product Information for anti-TNFRSF19 antibody

Analysis of EST clones and use of a signal sequence trap screening of the murine brain have led to identification of a new member of the tumor necrosis factor receptor superfamily designated as TAJ and TROY, respectively (1,2). TAJ/TROY is a membrane protein of 423 amino acids with characteristic cysteine-rich motifs in the extracellular domain and a tumor necrosis factor receptor-associated factor 2 (TRAF2) binding sequence in the cytoplasmic domain. Human TROY has 78% identity at the amino acid level with mouse TROY. The extracellular domain of TROY has significant homology with that of EDAR (another newly identified receptor belonging to TNF receptor family) that specifies hair follicle fate (3). The cytoplasmic domain of TAJ is different from other TNF receptor family members. Although its cytoplasmic domain does not contain death domain, TAJ/TROY induced cell death is probably mediated through a caspase-independent pathway. TAJ-induced JNK activation was not blocked by dominant-negative inhibitors of TRAF2, TRAF5, or ASK1, which have been previously implicated in JNK activation via TNFR1 and CD40. However, co-immunoprecipitation assays revealed that TAJ is capable of binding a number of different TRAF family members, and it is possible that TAJ-induced JNK activation is mediated by an as yet untested TRAF homolog, such as TRAF6. TROY mRNA is highly expressed in brain, prostate and embryo and moderately expressed in the heart, lung, liver.

Product Categories/Family for anti-TNFRSF19 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 325120961
• NCBI GeneID: 55504
• NCBI Accession #: NP_001191388.1
• NCBI GenBank Nucleotide #: NM_001204459.1
• UniProt Accession #: Q9NS68; Q9BXZ9; Q9BY00; Q9NZV2; B1AM40; B1AM41; B4E2I6
• Molecular Weight: 46,015 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 19 isoform 3
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 19
• NCBI Official Symbol: TNFRSF19
• NCBI Official Synonym Symbols: TAJ; TROY; TRADE; TAJ-alpha
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 19; toxicity and JNK inducer
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 19
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: TNFRSF19
• UniProt Synonym Gene Names: TAJ; TROY
• UniProt Entry Name: TNR19_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is highly expressed during embryonic development. It has been shown to interact with TRAF family members, and to activate JNK signaling pathway when overexpressed in cells. This receptor is capable of inducing apoptosis by a caspase-independent mechanism, and it is thought to play an essential role in embryonic development. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

TNFRSF19: Can mediate activation of JNK and NF-kappa-B. May promote caspase-independent cell death. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q12.11-q12.3

Cellular Component: integral to membrane

Molecular Function: tumor necrosis factor receptor activity

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; tumor necrosis factor-mediated signaling pathway; hair follicle development; apoptosis; JNK cascade