Western Blot (WB)
(Wesrern Blot of yeast during G1 arrest (Sic1 stable) and telophase arrest (Sic1 unstable))
Rat Sic1 Monoclonal Antibody | anti-Sic1 antibody
Sic1 Rat Monoclonal Antibody - Purified
Reactivity
Yeast, other species have not been tested.
Synonyms
Sic1; Monoclonal Antibody; Sic1 Rat Monoclonal Antibody - Purified; anti-Sic1 antibody
Host
Rat
Reactivity
Yeast, other species have not been tested.
Clonality
Monoclonal
Isotype
IgG2a kappa
Clone Number
6B4
Specificity
The antibody recognizes the Sic1 protein in yeast synchronized in G1 phase, and is negative for yeast synchronized in M phase.
Form/Format
Lyophilized protein G purified in PBS pH7.4
Immunogen
Sic1 recombinant protein
Target
Sic1
Target background
Sic1 participates in cell-cycle regulation of budding yeast, Saccharomyces cerevisiae. The protein acts as a stoichiometric inhibitor that binds to B-type cyclin-Cdk1 complexes, which, through precise temporal activation and inactivation, drive progression through the cell cycle. Multi-site phosphorylation of Sic1 leading to ubiquitination and destruction of the protein activates the Clb5,6-Cdk1 complex and promotes entry into from the G1 (Gap1) into the S (Synthesis) phase of the cell cycle, during which DNA is replicated. Mutants of Sic1 demonstrate a delay shifting from anaphase to telophase.
Target alias
SDB25, BYC1
Preservative
None
Preparation and Storage
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage. To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (1 year lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible wit your assay.
Western Blot (WB)
(Wesrern Blot of yeast during G1 arrest (Sic1 stable) and telophase arrest (Sic1 unstable))
Western Blot (WB)
(Wesrern Blot of yeast during G1 arrest (Sic1 stable) and telophase arrest (Sic1 unstable))
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Product Notes
The Sic1 (Catalog #AAA588350) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Sic1 Rat Monoclonal Antibody - Purified reacts with Yeast, other species have not been tested. and may cross-react with other species as described in the data sheet. It is sometimes possible for the material contained within the vial of "Sic1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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