Testing Data
(IF staining of U251 cells using antibodyMBS6008385. Cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with antibody MBS6008385 (1:25 dilution; 1 h at 37 degree C). Alexa Fluor 488-conjugated donkey anti-rabbit secondary antibody (green) was used (1:400, 50 min at 37 degree C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1h at 37 degree C). CACNA2D2 immunoreactivity is localized significantly to cytoplasm. )
Rabbit anti-Human, Mouse CACNA2D2 Polyclonal Antibody | anti-CACNA2D2 antibody
CACNA2D2, ID (CACNA2D2, KIAA0558, Voltage-dependent calcium channel subunit alpha-2/delta-2, Voltage-gated calcium channel subunit alpha-2/delta-2, Voltage-dependent calcium channel subunit alpha-2-2, Voltage-dependent calcium channel subunit delta-2) (AP
FLISA: 1:1,000
WB: 1:1,000
Applications are based on unconjugated antibody.
Testing Data
(IF staining of U251 cells using antibodyMBS6008385. Cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with antibody MBS6008385 (1:25 dilution; 1 h at 37 degree C). Alexa Fluor 488-conjugated donkey anti-rabbit secondary antibody (green) was used (1:400, 50 min at 37 degree C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1h at 37 degree C). CACNA2D2 immunoreactivity is localized significantly to cytoplasm. )
Testing Data
(IF staining of U251 cells using antibodyMBS6008385. Cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with antibody MBS6008385 (1:25 dilution; 1 h at 37 degree C). Alexa Fluor 488-conjugated donkey anti-rabbit secondary antibody (green) was used (1:400, 50 min at 37 degree C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1h at 37 degree C). CACNA2D2 immunoreactivity is localized significantly to cytoplasm. )
Western Blot (WB)
(Western blot analysis of CACNA2D2 in mouse kidney tissue lysate (35ug/lane) using antibodyMBS6008385. )
Western Blot (WB)
(Western blot analysis of CACNA2D2 in mouse kidney tissue lysate (35ug/lane) using antibodyMBS6008385. )
Immunohistochemistry (IHC)
(IHC staining of CACNA2D2 in formalin-fixed, paraffi-embedded human cerebellum tissue using antibodyMBS6008385. Peroxidase-conjugated secondary antibody and DAB staining were used for visualization. )
Immunohistochemistry (IHC)
(IHC staining of CACNA2D2 in formalin-fixed, paraffi-embedded human cerebellum tissue using antibodyMBS6008385. Peroxidase-conjugated secondary antibody and DAB staining were used for visualization. )
NCBI and Uniprot Product Information
NCBI Description
Calcium channels mediate the entry of calcium ions into the cell upon membrane polarization. This gene encodes the alpha-2/delta subunit of the voltage-dependent calcium channel complex. The complex consists of the main channel-forming subunit alpha-1, and auxiliary subunits alpha-2/delta, beta, and gamma. The auxiliary subunits function in the assembly and membrane localization of the complex, and modulate calcium currents and channel activation/inactivation kinetics. The subunit encoded by this gene undergoes post-translational cleavage to yield the extracellular alpha2 peptide and a membrane-anchored delta polypeptide. This subunit is a receptor for the antiepileptic drug, gabapentin. Mutations in this gene are associated with early infantile epileptic encephalopathy. Single nucleotide polymorphisms in this gene are correlated with increased sensitivity to opioid drugs. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2014]
Uniprot Description
CACNA2D2: The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Acts as a regulatory subunit for P/Q-type calcium channel (CACNA1A), N-type (CACNA1B), L-type (CACNA1C OR CACNA1D) and possibly T-type (CACNA1G). Overexpression induces apoptosis. Belongs to the calcium channel subunit alpha-2/delta family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 3p21.3
Cellular Component: plasma membrane; voltage-gated calcium channel complex
Molecular Function: voltage-gated calcium channel activity; metal ion binding
Biological Process: rhythmic synaptic transmission; energy reserve metabolic process; regulation of multicellular organism growth; muscle fiber development; positive regulation of organ growth; regulation of insulin secretion; neuromuscular junction development
Research Articles on CACNA2D2
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Product Notes
The CACNA2D2 cacna2d2 (Catalog #AAA6280310) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CACNA2D2, ID (CACNA2D2, KIAA0558, Voltage-dependent calcium channel subunit alpha-2/delta-2, Voltage-gated calcium channel subunit alpha-2/delta-2, Voltage-dependent calcium channel subunit alpha-2-2, Voltage-dependent calcium channel subunit delta-2) (AP reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CACNA2D2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB), Immunofluorescence (IF). IHC: 1:10-50 FLISA: 1:1,000 WB: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CACNA2D2 cacna2d2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CACNA2D2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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