Western Blot (WB)
(Western blot analysis of anti-NEK4 Pab in Y79 cell line lysates (35ug/lane). NEK4 (arrow) was detected using the purified Pab.)
Rabbit anti-Human, mouse NEK4 Polyclonal Antibody | anti-NEK4 antibody
NEK4 Antibody (C-term)
Gene Names
NEK4; NRK2; STK2; pp12301
Reactivity
Human, mouse
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
NEK4; Polyclonal Antibody; NEK4 Antibody (C-term); Serine/threonine-protein kinase Nek4; Never in mitosis A-related kinase 4; NimA-related protein kinase 4; Serine/threonine-protein kinase 2; Serine/threonine-protein kinase NRK2; STK2; anti-NEK4 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This NEK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 793-824 amino acids from the C-terminal region of human NEK4.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
793-824
Sequence Length
752
Applicable Applications for anti-NEK4 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of anti-NEK4 Pab in Y79 cell line lysates (35ug/lane). NEK4 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-NEK4 Pab in Y79 cell line lysates (35ug/lane). NEK4 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Related Product Information for anti-NEK4 antibody
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
Product Categories/Family for anti-NEK4 antibody
References
Levedakou, E.N., et al., Oncogene 9(7):1977-1988 (1994).
Lu, K.P., et al., Prog Cell Cycle Res 1, 187-205 (1995).
Lu, K.P., et al., Prog Cell Cycle Res 1, 187-205 (1995).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
94597
NCBI Official Full Name
serine/threonine-protein kinase Nek4 isoform 2
NCBI Official Synonym Full Names
NIMA-related kinase 4
NCBI Official Symbol
NEK4
NCBI Official Synonym Symbols
NRK2; STK2; pp12301
NCBI Protein Information
serine/threonine-protein kinase Nek4
UniProt Protein Name
Serine/threonine-protein kinase Nek4
Protein Family
UniProt Gene Name
NEK4
UniProt Synonym Gene Names
STK2; NimA-related protein kinase 4
UniProt Entry Name
NEK4_HUMAN
Uniprot Description
NEK4: a protein kinase of the NEK family. Highly conserved between the mouse and human and expressed at high levels in the testis.
Protein type: Kinase, protein; Protein kinase, Other; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Other group; NEK family
Chromosomal Location of Human Ortholog: 3p21.1
Cellular Component: cytoplasm; cilium
Molecular Function: protein serine/threonine kinase activity; manganese ion binding; ATP binding; receptor signaling protein serine/threonine kinase activity
Biological Process: mitosis; cell division; mitotic cell cycle; protein amino acid phosphorylation; response to DNA damage stimulus
Research Articles on NEK4
1. Nek4 as a novel regulator of replicative senescence and the response to double-stranded DNA damage.
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Product Notes
The NEK4 nek4 (Catalog #AAA9211944) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 793-824. The NEK4 Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NEK4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the NEK4 nek4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEK4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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