Rabbit Synapsin I Polyclonal Antibody | anti-SYN1 antibody

Anti-Synapsin I

Cat. Num. AAA502117

• Reactivity: Human, mouse, rat
• Applications: Western Blot, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the native protein was coupled.)
• Synonyms: Synapsin I; Polyclonal Antibody; Anti-Synapsin I; anti-SYN1 antibody

• Host: Rabbit
• Reactivity: Human, mouse, rat
• Clonality: Polyclonal
• Specificity: Specific for the ~78k synapsin I doublet in Western blots of rat brain extracts. Immunolabeling blocked by preadsorption of antibody with the protein used to generate the antibody.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the native protein was coupled.)
• Form/Format: 10 ug antibody vial; lyophilized from 5 mM ammonium bicarbonate. The antibody should be reconstituted in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution the antibody sh
• Sequence Length: 706

• Applicable Applications for anti-SYN1 antibody: Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000; IHC: 1:1000; IF: 1:1000; IP: 1 ug per 200 ug lysate
• Antigen: Native protein purified from bovine brain.
• Immunogen Information: Native protein purified from bovine brain
• Immunogen Species: bovine
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat, mouse and human tissue.
• Biological Significance: Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation (Moore and Bernstein, 1989; Stone et al., 1994). Thus, synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002).
• Preparation and Storage: For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year

Testing Data

Related Product Information for anti-SYN1 antibody

Affinity purified rabbit polyclonal antibody

References

• Feng J, Chi P, Blanpied TA, Xu YM, Magarinos AM, Ferreira A, Takahashi RH, Kao HT, McEwen BS, Ryan TA, Augustine GJ, Greengard P (2002) Regulation of neurotransmitter release by synapsin III. J Neurosci 22:4372-4380.
• Jovanovic JN, Sihra TS, Nairn AC, Hemmings HC, Jr., Greengard P, Czernik AJ (2001) Opposing changes in phosphorylation of specific sites in synapsin I during Ca2+-dependent glutamate release in isolated nerve terminals. J Neurosci 21:7944-7953.
• Kao HT, Song HJ, Porton B, Ming GL, Hoh J, Abraham M, Czernik AJ, Pieribone VA, Poo MM, Greengard P (2002) A protein kinase A-dependent molecular switch in synapsins regulates neurite outgrowth. Nature Neurosci 5:431-437.
• Moore RY, Bernstein M (1989) Synaptogenesis in the rat suprachiasmatic nucleus demonstrated by electron microscopy and synapsin I immunoreactivity. J Neurosci 9:2151-2162
• Nayak AS, Moore CI, Browning MD (1996) CaM kinase II phosphorylation of the presynaptic protein synapsin is persistently increased during expression of long-term potentiation. Proc Natl Acad Sci (USA) 93:15451-15456.

NCBI and Uniprot Product Information

• NCBI GI #: 27806393
• NCBI GeneID: 281510
• NCBI Accession #: NP_776616.1
• NCBI GenBank Nucleotide #: NM_174191.2
• UniProt Accession #: P17599
• Molecular Weight: 78
• NCBI Official Full Name: synapsin-1
• NCBI Official Symbol: SYN1
• NCBI Protein Information: synapsin-1
• UniProt Protein Name: Synapsin-1
• Protein Family: Synapsin
• UniProt Gene Name: SYN1
• UniProt Entry Name: SYN1_BOVIN

Uniprot Description

Function: Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level

By similarity.

Subunit structure: Homodimer. Interacts with CAPON. Forms a ternary complex with NOS1. Isoform Ibinteracts with PRNP

By similarity.

Subcellular location: Cell junction › synapse. Golgi apparatus

By similarity.

Domain: The A region binds phospholipids with a preference for negatively charged species

By similarity.

Post-translational modification: Substrate of at least four different protein kinases. Phosphorylation may play a role in the regulation of synapsin-1 in the nerve terminal.Phosphorylation at Ser-9 dissociates synapsins from synaptic vesicles

By similarity.

Sequence similarities: Belongs to the synapsin family.