Rabbit anti-Mouse, rat NMDA NR1 Subunit, Splice Variant N1 Polyclonal Antibody | anti-GRIN1 antibody

Anti-NMDA Receptor, NR1 Subunit, N1 Splice Variant Insert

Cat. Num. AAA502082

• Gene Names: Grin1; NR1; GluN1; NMDAR1
• Reactivity: Mouse, rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.)
• Synonyms: NMDA NR1 Subunit; Splice Variant N1; Polyclonal Antibody; Anti-NMDA Receptor; NR1 Subunit; N1 Splice Variant Insert; anti-GRIN1 antibody

• Host: Rabbit
• Reactivity: Mouse, rat
• Clonality: Polyclonal
• Specificity: Specific for the ~120k NR1 subunit, N1 splice variant insert protein of the NMDA receptor. Does not recognize NR1 subunits of the NMDA receptor that do not contain the N1 insert.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.)
• Form/Format: 25 ug antibody vial; lyophilized from 5 mM ammonium bicarbonate. The antibody should be reconstituted in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution the antibody sh
• Sequence Length: 959

• Applicable Applications for anti-GRIN1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000; IHC (frozen sections; unpublished observations): 1:1000 to 1:2000
• Antigen: Peptide from the NR1 subunit, N1 splice variant insert of rat NMDA receptor.
• Immunogen Information: Synthetic peptide corresponding to amino acid residues specific to the NR1 subunit, N1 splice variant insert conjugated to KLH
• Immunogen Species: Rat
• Reactivity Assumed Based on 100% Sequence Homology: Canine, chicken, human, non-human primates and Xenopus
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots in rat and mouse tissues. It is anticipated that the antibody will also work with canine, chicken, human, non-human primate and Xenopus tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA Receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). There are a number of different splice variants of the NR1-Subunit (Foldes et al., 1994; Zukin and Bennett, 1995). Differential splicing of three exons in the NR1-Subunit generates up to eight NR1-Subunit splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2'). Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR.
• Preparation and Storage: For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Testing Data

Related Product Information for anti-GRIN1 antibody

Affinity purified rabbit polyclonal antibody

References

• Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends Neurosci 25:571-577.
• Foldes RL, Rampersad V, Kamboj RK (1994) Cloning and sequence analysis of additional splice variants encoding human N-methyl-D-aspartate receptor (hNR1) subunits. Gene 147:303-304.
• Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33
• Wenthold RJ, Prybylowski K, Standley S, Sans N, Petralia RS (2003) Trafficking of NMDA receptors. Annu Rev Pharmacol Toxicol 43:335-358.
• Zukin RS, Bennett MVL (1995) Alternatively spliced isoforms of the NMDARI receptor subunit. Trends Neurosci 18:306-313.

NCBI and Uniprot Product Information

• NCBI GI #: 396578144
• NCBI GeneID: 24408
• NCBI Accession #: NP_001257531.1
• NCBI GenBank Nucleotide #: NM_001270602.1
• UniProt Accession #: P35439; Q62646
• Molecular Weight: 120
• NCBI Official Full Name: glutamate receptor ionotropic, NMDA 1 isoform 1b
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, N-methyl D-aspartate 1
• NCBI Official Symbol: Grin1
• NCBI Official Synonym Symbols: NR1; GluN1; NMDAR1
• NCBI Protein Information: glutamate receptor ionotropic, NMDA 1; NMD-R1; neurotransmitter receptor; NMDA R1 receptor C1 cassette; N-methyl-D-aspartate glutamate receptor; glutamate [NMDA] receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1
• UniProt Protein Name: Glutamate receptor ionotropic, NMDA 1
• Protein Family: Glutamate receptor ionotropic
• UniProt Gene Name: Grin1
• UniProt Synonym Gene Names: Nmdar1; GluN1; NMD-R1
• UniProt Entry Name: NMDZ1_RAT

NCBI Description

subunit of NMDA-preferring ionotropic glutamate receptors; may play a role in long term potentiation [RGD, Feb 2006]

Uniprot Description

Function: NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors. Ref.18

Subunit structure: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B); disulfide-linked

By similarity. Found in a complex with GRIN2A or GRIN2B, GRIN3A and PPP2CB. Found in a complex with GRIN2A or GRIN2B and GRIN3B. Interacts with SNX27 (via PDZ domain); the interaction is required for recycling to the plasma membrane when endocytosed and prevent degradation in lysosomes

By similarity. Interacts with DLG4 and MPDZ. Interacts with LRFN1 and LRFN2. Interacts with MYZAP. Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.13 Ref.14 Ref.15 Ref.17

Subcellular location: Cell membrane; Multi-pass membrane protein

By similarity. Cell junction › synapse › postsynaptic cell membrane

By similarity. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density

By similarity. Note: Enriched in postsynaptic plasma membrane and postsynaptic densities

By similarity.

Post-translational modification: NMDA is probably regulated by C-terminal phosphorylation of an isoform ofNR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity. Ref.7 Ref.12

Sequence similarities: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily. [View classification]