Rabbit NMDA NR2A Subunit Polyclonal Antibody | anti-GRIN2A antibody

Anti-NMDA Receptor, NR2A Subunit

Cat. Num. AAA502083

• Gene Names: Grin2a; NR2A; GluN2A; NMDAR2A
• Reactivity: Rat, mouse, human
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.)
• Synonyms: NMDA NR2A Subunit; Polyclonal Antibody; Anti-NMDA Receptor; NR2A Subunit; anti-GRIN2A antibody

• Host: Rabbit
• Reactivity: Rat, mouse, human
• Clonality: Polyclonal
• Specificity: Specific for the ~180k NR2A subunit of the NMDA receptor. Recognizes human, mouse and rat forms of the NR2A subunit of NMDAR. No reactivity towards the NR2B and NR2C subunits. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.)
• Form/Format: 10 ug antibody vial; lyophilized from 5 mM ammonium bicarbonate. The antibody should be reconstituted in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution the antibody sh
• Sequence Length: 1464

• Applicable Applications for anti-GRIN2A antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000; IHC: 1:1000; IP: 3 ul per 200 ug lysate
• Antigen: Fusion protein from the C-terminus of the NR2A subunit of rat NMDA receptor
• Immunogen Information: Fusion protein from the C-terminal region of the NR2A subunit
• Immunogen Species: Rat
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with human, mouse and rat tissue.
• Biological Significance: The ion channels activated by glutamate are typically divided into two classes. Glutamate receptors that are activated by kainate and alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.
• Preparation and Storage: Store at -20 degree C; stable for at least 1 year

Testing Data

Related Product Information for anti-GRIN2A antibody

Affinity purified rabbit polyclonal antibody

References

• Alvestad RM, Grosshans DR, Coultrap SJ, Nakazawa T, Yamamoto T, Browning MD (2003) Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. J Biol Chem 278:11020-11025.
• Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends Neurosci 25:571-577.
• Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33.
• Lovinger DM, White G, Weight FF (1989) Ethanol inhibits NMDA-activated ion current in hippocampal neurons. Science 243:1721-1724.
• Lu W-Y, Xiong Z-G, Lei S, Orser BA, Browning MD, MacDonald JF (1999) G-protein coupled receptors act via protein kinase C and Src to regulate NMDA receptors. Nature Neurosci 2:331-338.

NCBI and Uniprot Product Information

• NCBI GI #: 140971205
• NCBI GeneID: 24409
• NCBI Accession #: NP_036705.3
• NCBI GenBank Nucleotide #: NM_012573.3
• UniProt Accession #: Q00959; O08948; Q63728
• Molecular Weight: 180
• NCBI Official Full Name: glutamate receptor ionotropic, NMDA 2A
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, N-methyl D-aspartate 2A
• NCBI Official Symbol: Grin2a
• NCBI Official Synonym Symbols: NR2A; GluN2A; NMDAR2A
• NCBI Protein Information: glutamate receptor ionotropic, NMDA 2A; N-methyl D-aspartate receptor subtype 2A; N-methyl-D-aspartate receptor subunit 2A; glutamate [NMDA] receptor subunit epsilon-1
• UniProt Protein Name: Glutamate receptor ionotropic, NMDA 2A
• Protein Family: Glutamate receptor ionotropic
• UniProt Gene Name: Grin2a
• UniProt Synonym Gene Names: GluN2A; NMDAR2A; NR2A
• UniProt Entry Name: NMDE1_RAT

NCBI Description

may play a role in synaptic transmission, learning and memory [RGD, Feb 2006]

Uniprot Description

NMDAR2A: a subunit of N-methyl-D-aspartate (NMDA) receptors, members of the glutamate receptor channel superfamily. Possesses high calcium permeability and voltage-dependent sensitivity to magnesium and is modulated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Channel, ligand-gated

Cellular Component: presynaptic membrane; postsynaptic membrane; synaptic vesicle; neuron projection; cell surface; membrane; endoplasmic reticulum; postsynaptic density; dendritic spine; terminal button; synapse; cell junction; N-methyl-D-aspartate selective glutamate receptor complex

Molecular Function: voltage-gated cation channel activity; protein dimerization activity; neurotransmitter binding; glutamate receptor binding; zinc ion binding; calcium channel activity; protein kinase binding; ATPase binding; protein binding; extracellular-glutamate-gated ion channel activity; glutamate binding; protein heterodimerization activity; ionotropic glutamate receptor activity; N-methyl-D-aspartate selective glutamate receptor activity; protein complex binding; cell adhesion molecule binding; cation channel activity; receptor binding

Biological Process: regulation of long-term neuronal synaptic plasticity; startle response; positive regulation of apoptosis; rhythmic process; regulation of synaptic plasticity; response to other organism; sensory perception of pain; response to organic cyclic substance; dopamine metabolic process; response to carbohydrate stimulus; synaptic transmission; protein localization; learning and/or memory; calcium ion transport; response to methylmercury; response to wounding; visual learning; locomotion; cation transport; serotonin metabolic process; protein tetramerization; regulation of synaptic transmission; response to drug; regulation of action potential; synaptic transmission, glutamatergic; response to light stimulus; hippocampus development; response to amphetamine; learning; sleep; response to cocaine; memory; response to manganese ion; detection of mechanical stimulus involved in sensory perception of pain; regulation of membrane potential; regulation of postsynaptic membrane potential; response to ethanol; neurogenesis; spinal cord development; directional locomotion; ionotropic glutamate receptor signaling pathway; regulation of sensory perception of pain; cerebral cortex development; negative regulation of protein catabolic process; response to calcium ion; regulation of excitatory postsynaptic membrane potential; response to amine stimulus