Rabbit anti-Human Syk Polyclonal Antibody | anti-Syk antibody

Anti-Syk Antibody

Cat. Num. AAA417316

• Gene Names: Syk; Sykb
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity purified
• Synonyms: Syk; Polyclonal Antibody; Anti-Syk Antibody; Anti-Syk (Ab-323) Antibody; anti-Syk antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Syk (Ab-323) Antibody detects endogenous levels of total syk protein.
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 629

• Applicable Applications for anti-Syk antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; IF:1:100~1:200
• Storage Buffer: Rabbit IgG in phosphate buffered saline (without Mg< sup>2< /sup>+ and Ca< sup>2< /sup>+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Immunogen: The antibody was produced against synthesized non-phosphopeptide derived from human syk around the phosphorylation site of tyrosine 323 (N-P-YP-E-P).
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-Syk antibody

This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

NCBI and Uniprot Product Information

• NCBI GI #: 818861
• NCBI GeneID: 20963
• Molecular Weight: 71,376 Da
• NCBI Official Full Name: SYK
• NCBI Official Synonym Full Names: spleen tyrosine kinase
• NCBI Official Symbol: Syk
• NCBI Official Synonym Symbols: Sykb
• NCBI Protein Information: tyrosine-protein kinase SYK
• UniProt Protein Name: Tyrosine-protein kinase SYK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: Syk
• UniProt Entry Name: KSYK_MOUSE

Uniprot Description

Syk: a cytoplasmic tyrosine kinase of the SYK family containing two SH2 domains. Plays a central role in the B cell receptor (BCR) response. An upstream activator of the PI3K, PLCgamma2, and Rac/cdc42 pathways in the BCR response. Required for the sequential events of Fc gamma IIa receptor-mediated phagocytosis. Expression highest in murine spleen, heart, mammary gland and thymus. Two splice variant isoforms have been described.

Protein type: Kinase, protein; Protein kinase, TK; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); TK group; Syk family

Cellular Component: T cell receptor complex; extrinsic to internal side of plasma membrane; protein complex; membrane; cell; cytoplasm; plasma membrane; intracellular; cytoplasmic vesicle; nucleus; B cell receptor complex

Molecular Function: protein domain specific binding; nucleotide binding; non-membrane spanning protein tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; protein kinase binding; protein kinase activity; integrin binding; transferase activity; protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; kinase activity; ATP binding; receptor binding

Biological Process: peptidyl-tyrosine phosphorylation; activation of MAPK activity; transcription factor import into nucleus; protein amino acid autophosphorylation; positive regulation of interleukin-3 biosynthetic process; positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process; positive regulation of calcium-mediated signaling; regulation of phagocytosis; protein amino acid phosphorylation; enzyme linked receptor protein signaling pathway; activation of JNK activity; serotonin secretion; leukocyte adhesion; B cell receptor signaling pathway; cell surface receptor linked signal transduction; regulation of transcription factor activity; positive regulation of bone resorption; beta selection; angiogenesis; positive regulation of gamma-delta T cell differentiation; integrin-mediated signaling pathway; neutrophil chemotaxis; regulation of immune response; cell migration; adaptive immune response; regulation of superoxide release; immune system process; positive regulation of cell adhesion mediated by integrin; positive regulation of mast cell degranulation; regulation of neutrophil degranulation; leukotriene biosynthetic process; G-protein coupled receptor protein signaling pathway; peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; neutrophil activation during immune response; leukocyte activation during immune response; serotonin secretion by platelet; defense response to bacterium; macrophage activation during immune response; positive regulation of B cell differentiation; innate immune response; blood vessel morphogenesis; positive regulation of alpha-beta T cell proliferation; lymph vessel development; phosphorylation; positive regulation of alpha-beta T cell differentiation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cytokine secretion