Rabbit anti-Human Syk Polyclonal Antibody | anti-Sykb antibody

Syk, phosphorylated (Tyr323)

Cat. Num. AAA630492

• Gene Names: Sykb; Syk
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by protein A and peptide affinity chromatography.
• Synonyms: Syk; Polyclonal Antibody; phosphorylated (Tyr323); Anti -Syk; anti-Sykb antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects overexpressed levels of Tyr323 phosphorylated Syk. It does not crossreact with other tyrosinephosphorylated members of the Syk/Zap-70 tyrosine kinase family.
• Purity/Purification: Affinity Purified; Purified by protein A and peptide affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.

• Applicable Applications for anti-Sykb antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation.; Dilution: Western Blot: 1:1000; Immunoprecipitation: 1:50
• Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr323 of human Syk or Tyr317 of mouse Syk.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis of extracts from 293T cells expressing recombinant wild-type or mutant Syk proteins, cotransfected with CD8, using MBS630492 (upper) or S9100-01 (lower). (Provided by Dr. Alagarsamy L. Reddi, laboratory of Dr. Hamid Band, Harvard University, Massachusetts.))

Related Product Information for anti-Sykb antibody

Syk, one of the well known protein tyrosine kinases, is widely expressed and plays an important role in intracellular signal transduction in hematopoietic cells (1-3). Syk has been shown to interact with immunoreceptor tyrosine-based activation motifs (ITAMs) located in the cytoplasmic domains of immune receptors (4). It couples the activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation and phagocytosis (4). There is also evidence of a role for Syk in nonimmune cells, and Syk may be a potential tumor suppressor in human breast carcinomas (5). Tyr323 is a negative regulatory phosphorylation site within the SH2-kinase linker region in Syk. Phosphorylation of Tyr323 provides a direct binding site to the TKB domain of Cbl (6,7).

Product Categories/Family for anti-Sykb antibody

Antibodies; Abs to Tyrosine Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 818861
• NCBI GeneID: 20963
• UniProt Accession #: P48025; Q3UPF7; Q3V3Q2; Q6P1E0
• Molecular Weight: 71,376 Da
• NCBI Official Full Name: SYK
• NCBI Official Synonym Full Names: spleen tyrosine kinase
• NCBI Official Symbol: Sykb
• NCBI Official Synonym Symbols: Syk
• NCBI Protein Information: tyrosine-protein kinase SYK; OTTMUSP00000041832
• UniProt Protein Name: Tyrosine-protein kinase SYK
• UniProt Gene Name: Syk
• UniProt Synonym Gene Names: Sykb
• UniProt Entry Name: KSYK_MOUSE

Uniprot Description

Syk: a cytoplasmic tyrosine kinase of the SYK family containing two SH2 domains. Plays a central role in the B cell receptor (BCR) response. An upstream activator of the PI3K, PLCgamma2, and Rac/cdc42 pathways in the BCR response. Required for the sequential events of Fc gamma IIa receptor-mediated phagocytosis. Expression highest in murine spleen, heart, mammary gland and thymus. Two splice variant isoforms have been described.

Protein type: EC 2.7.10.2; Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); TK group; Syk family

Cellular Component: T cell receptor complex; extrinsic to internal side of plasma membrane; protein complex; membrane; cell; cytoplasm; plasma membrane; cytoplasmic vesicle; intracellular; nucleus; B cell receptor complex

Molecular Function: protein domain specific binding; nucleotide binding; non-membrane spanning protein tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; protein kinase binding; protein kinase activity; integrin binding; transferase activity; protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; kinase activity; ATP binding; receptor binding

Biological Process: peptidyl-tyrosine phosphorylation; transcription factor import into nucleus; activation of MAPK activity; protein amino acid autophosphorylation; positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process; positive regulation of interleukin-3 biosynthetic process; positive regulation of calcium-mediated signaling; regulation of phagocytosis; protein amino acid phosphorylation; enzyme linked receptor protein signaling pathway; activation of JNK activity; serotonin secretion; B cell receptor signaling pathway; leukocyte adhesion; cell surface receptor linked signal transduction; regulation of transcription factor activity; positive regulation of bone resorption; beta selection; positive regulation of gamma-delta T cell differentiation; angiogenesis; integrin-mediated signaling pathway; neutrophil chemotaxis; cell migration; adaptive immune response; regulation of immune response; immune system process; regulation of superoxide release; positive regulation of cell adhesion mediated by integrin; positive regulation of mast cell degranulation; regulation of neutrophil degranulation; leukotriene biosynthetic process; G-protein coupled receptor protein signaling pathway; peptidyl-serine phosphorylation; neutrophil activation during immune response; positive regulation of peptidyl-tyrosine phosphorylation; leukocyte activation during immune response; serotonin secretion by platelet; defense response to bacterium; macrophage activation during immune response; positive regulation of B cell differentiation; blood vessel morphogenesis; innate immune response; positive regulation of alpha-beta T cell proliferation; lymph vessel development; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of alpha-beta T cell differentiation; positive regulation of cytokine secretion