Rabbit anti-Human Syk Polyclonal Antibody | anti-Sykb antibody

Syk, phosphorylated (Tyr323)

Cat. Num. AAA627887

• Gene Names: Sykb; Syk
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Serum; Serum
• Synonyms: Syk; Polyclonal Antibody; phosphorylated (Tyr323); Anti -Syk; anti-Sykb antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes endogenous levels of human syk only when phosphorylated at tyrosine 323.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-Sykb antibody: Immunohistochemistry (IHC)
• Application Notes: Dilution: Immunohistochemistry: 1:50-100
• Immunogen: Synthesized phosphopeptide derived from human syk around the phosphorylation site of tyrosine 323 (N-P-YP-E-P).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MBS627887.)

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed Hela cells using MBS627887.)

Western Blot (WB)

(Western blot analysis of extracts from JK cells untreated or treated with H2O2 for the indicated times, using MBS627887.)

Related Product Information for anti-Sykb antibody

Syk protein is expressed in immature T cells, all B cells, basophils, hepatocytes, platelets, and erythrocytes. It functions in some T cell receptor signalling processes in addition to B cell receptor and integrin-mediated signal transduction processes.

Product Categories/Family for anti-Sykb antibody

Antibodies; Abs to Tyrosine Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 818861
• NCBI GeneID: 20963
• UniProt Accession #: P48025; Q3UPF7; Q3V3Q2; Q6P1E0
• Molecular Weight: 71,376 Da
• NCBI Official Full Name: SYK
• NCBI Official Synonym Full Names: spleen tyrosine kinase
• NCBI Official Symbol: Sykb
• NCBI Official Synonym Symbols: Syk
• NCBI Protein Information: tyrosine-protein kinase SYK; OTTMUSP00000041832
• UniProt Protein Name: Tyrosine-protein kinase SYK
• UniProt Gene Name: Syk
• UniProt Synonym Gene Names: Sykb
• UniProt Entry Name: KSYK_MOUSE

Uniprot Description

Syk: a cytoplasmic tyrosine kinase of the SYK family containing two SH2 domains. Plays a central role in the B cell receptor (BCR) response. An upstream activator of the PI3K, PLCgamma2, and Rac/cdc42 pathways in the BCR response. Required for the sequential events of Fc gamma IIa receptor-mediated phagocytosis. Expression highest in murine spleen, heart, mammary gland and thymus. Two splice variant isoforms have been described.

Protein type: Protein kinase, tyrosine (non-receptor); Protein kinase, TK; Kinase, protein; EC 2.7.10.2; TK group; Syk family

Cellular Component: T cell receptor complex; extrinsic to internal side of plasma membrane; protein complex; membrane; cell; cytoplasm; plasma membrane; intracellular; cytoplasmic vesicle; nucleus; B cell receptor complex

Molecular Function: protein domain specific binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; receptor signaling protein tyrosine kinase activity; protein kinase binding; protein kinase activity; transferase activity; integrin binding; protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; kinase activity; ATP binding; receptor binding

Biological Process: peptidyl-tyrosine phosphorylation; activation of MAPK activity; transcription factor import into nucleus; protein amino acid autophosphorylation; positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process; positive regulation of interleukin-3 biosynthetic process; positive regulation of calcium-mediated signaling; regulation of phagocytosis; enzyme linked receptor protein signaling pathway; protein amino acid phosphorylation; activation of JNK activity; serotonin secretion; B cell receptor signaling pathway; leukocyte adhesion; cell surface receptor linked signal transduction; regulation of transcription factor activity; positive regulation of bone resorption; beta selection; positive regulation of gamma-delta T cell differentiation; angiogenesis; integrin-mediated signaling pathway; neutrophil chemotaxis; adaptive immune response; regulation of immune response; cell migration; regulation of superoxide release; immune system process; positive regulation of cell adhesion mediated by integrin; positive regulation of mast cell degranulation; regulation of neutrophil degranulation; leukotriene biosynthetic process; G-protein coupled receptor protein signaling pathway; neutrophil activation during immune response; peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; serotonin secretion by platelet; leukocyte activation during immune response; macrophage activation during immune response; defense response to bacterium; innate immune response; blood vessel morphogenesis; positive regulation of B cell differentiation; positive regulation of alpha-beta T cell proliferation; lymph vessel development; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of alpha-beta T cell differentiation; positive regulation of cytokine secretion