Rabbit anti-Human, Mouse Syk Polyclonal Antibody | anti-Sykb antibody

Syk, phosphorylated (Tyr525/526)

Cat. Num. AAA624263

• Gene Names: Sykb; Syk
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Syk; Polyclonal Antibody; phosphorylated (Tyr525/526); Anti -Syk; anti-Sykb antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects overexpressed levels of Syk protein only when phosphorylated at Tyr525/526 of human Syk or Tyr519/ 520 of mouse Syk. Does not crossreact with other tyrosine-phosphorylated members of the Syk/Zap-70 tyrosine kinase family.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

• Applicable Applications for anti-Sykb antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot and ELISA.; Dilution: Western Blot: 1:1000; ELISA: 1:1000
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Tyr525/526 of human Syk (KLH coupled).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from 293T cells expressing recombinant wild-type and mutant Syk proteins, cotransfected with CD8, using Phospho-Syk (Tyr525/526) (upper) or Syk MBS624263(lower). (Provided by Dr. Hamid Band, Harvard University, Massachusetts.))

Testing Data

(Dephosphorylation of phosphorylated Wild-type Syk abolishes Phospho-Syk (Tyr525/526) Antibody's recognition of this protein.)

Related Product Information for anti-Sykb antibody

Syk, one of the well known protein tyrosine kinases, is widely expressed and plays an important role in intracellular signal transduction in hematopoietic cells (1-3). Syk has been shown to interact with immunoreceptor tyrosine-based activation motifs (ITAMs) located in the cytoplasmic domains of immune receptors (4). It couples the activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation and phagocytosis (4). There is also evidence of a role for Syk in nonimmune cells, and Syk may be a potential tumor suppressor in human breast carcinomas (5). Phosphorylation of Tyr525/526 in the activation loop of human Syk (equivalent to Tyr519/520 of mouse Syk) is essential for Syk function (6).

Product Categories/Family for anti-Sykb antibody

Antibodies; Abs to Tyrosine Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 818861
• NCBI GeneID: 20963
• UniProt Accession #: P48025; Q3UPF7; Q3V3Q2; Q6P1E0
• Molecular Weight: 71,376 Da
• NCBI Official Full Name: SYK
• NCBI Official Synonym Full Names: spleen tyrosine kinase
• NCBI Official Symbol: Sykb
• NCBI Official Synonym Symbols: Syk
• NCBI Protein Information: tyrosine-protein kinase SYK; OTTMUSP00000041832
• UniProt Protein Name: Tyrosine-protein kinase SYK
• UniProt Gene Name: Syk
• UniProt Synonym Gene Names: Sykb
• UniProt Entry Name: KSYK_MOUSE

Uniprot Description

Syk: a cytoplasmic tyrosine kinase of the SYK family containing two SH2 domains. Plays a central role in the B cell receptor (BCR) response. An upstream activator of the PI3K, PLCgamma2, and Rac/cdc42 pathways in the BCR response. Required for the sequential events of Fc gamma IIa receptor-mediated phagocytosis. Expression highest in murine spleen, heart, mammary gland and thymus. Two splice variant isoforms have been described.

Protein type: Protein kinase, TK; Kinase, protein; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; TK group; Syk family

Cellular Component: T cell receptor complex; extrinsic to internal side of plasma membrane; protein complex; membrane; cell; cytoplasm; plasma membrane; intracellular; cytoplasmic vesicle; nucleus; B cell receptor complex

Molecular Function: protein domain specific binding; nucleotide binding; non-membrane spanning protein tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; protein kinase binding; protein kinase activity; transferase activity; integrin binding; protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; kinase activity; ATP binding; receptor binding

Biological Process: peptidyl-tyrosine phosphorylation; transcription factor import into nucleus; activation of MAPK activity; protein amino acid autophosphorylation; positive regulation of interleukin-3 biosynthetic process; positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process; positive regulation of calcium-mediated signaling; regulation of phagocytosis; enzyme linked receptor protein signaling pathway; protein amino acid phosphorylation; serotonin secretion; activation of JNK activity; leukocyte adhesion; B cell receptor signaling pathway; cell surface receptor linked signal transduction; regulation of transcription factor activity; positive regulation of bone resorption; beta selection; positive regulation of gamma-delta T cell differentiation; angiogenesis; integrin-mediated signaling pathway; neutrophil chemotaxis; regulation of immune response; cell migration; adaptive immune response; immune system process; regulation of superoxide release; positive regulation of cell adhesion mediated by integrin; positive regulation of mast cell degranulation; regulation of neutrophil degranulation; leukotriene biosynthetic process; G-protein coupled receptor protein signaling pathway; peptidyl-serine phosphorylation; neutrophil activation during immune response; positive regulation of peptidyl-tyrosine phosphorylation; serotonin secretion by platelet; leukocyte activation during immune response; macrophage activation during immune response; defense response to bacterium; blood vessel morphogenesis; innate immune response; positive regulation of B cell differentiation; positive regulation of alpha-beta T cell proliferation; lymph vessel development; phosphorylation; positive regulation of alpha-beta T cell differentiation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cytokine secretion