Rabbit anti-Human, mouse STEA2 Polyclonal Antibody | anti-STEAP2 antibody

STEA2 Antibody (Center)

Cat. Num. AAA9214345

• Gene Names: STEAP2; STMP; IPCA1; PUMPCn; STAMP1; PCANAP1
• Reactivity: Human, mouse
• Applications: ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: STEA2; Polyclonal Antibody; STEA2 Antibody (Center); Metalloreductase STEAP2; 1161-; Prostate cancer-associated protein 1; Protein up-regulated in metastatic prostate cancer; PUMPCn; Six-transmembrane epithelial antigen of prostate 2; SixTransMembrane protein of prostate 1; STEAP2; PCANAP1; STAMP1; anti-STEAP2 antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Clone Number: RB26961
• Specificity: This STEA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 229-258 amino acids from the Central region of human STEA2.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 226-253
• Sequence Length: 419

• Applicable Applications for anti-STEAP2 antibody: ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB)
• Application Notes: WB~~1:500; IHC-P~~1:50~100; FC~~1:10~50
• Antigen Type: Synthetic Peptide
• Crown Antibody: Yes
• Function: Metalloreductase that has the ability to reduce both Fe(3+) to Fe(2+) and Cu(2+) to Cu(1+). Uses NAD(+) as acceptor (By similarity).
• Cellular Location: Endosome membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein
• Tissue Location: Expressed at high levels in prostate and at significantly lower levels in heart, brain, kidney, pancreas, and ovary.
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of lysates from 293, HepG2 cell line, mouse spleen tissue lysate (from left to right), using STEA2 Antibody (Center). MBS9214345 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

Western Blot (WB)

(Western blot analysis of extracts from 293 (lanes 1 and 2),HepG2 (lanes 3 and 4)cell line and mouse spleen (lanes 5 and 6)tissue lysate: 1, 3, 5. using STEA2 Antibody (Center)(MBS9214345), (1:500). 2, 4, 6. using STEA2 Antibody (Center), preincubated with the control peptide antigen.)

Immunohistochemistry (IHC)

(STEA2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the STEA2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(STEA2 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-STEAP2 antibody

This gene is a member of the STEAP family and encodes a
multi-pass membrane protein that localizes to the Golgi complex,
the plasma membrane, and the vesicular tubular structures in the
cytosol. A highly similar protein in mouse has both ferrireductase
and cupric reductase activity, and stimulates the cellular uptake
of both iron and copper in vitro. Increased transcriptional
expression of the human gene is associated with prostate cancer
progression. Alternate transcriptional splice variants, encoding
different isoforms, have been characterized.

Product Categories/Family for anti-STEAP2 antibody

Crown Antibodies; Cancer

References

Vaghjiani, R.J., et al. Tissue Eng Part A 15(8):2073-2083(2009)
Denoeud, F., et al. Genome Res. 17(6):746-759(2007)
Ohgami, R.S., et al. Blood 108(4):1388-1394(2006)

NCBI and Uniprot Product Information

• NCBI GI #: 100913194
• NCBI GeneID: 261729
• NCBI Accession #: NP_001035755.1; NP_001035756.1; NP_001231873.1; NP_001231875.1; NP_694544.2
• NCBI GenBank Nucleotide #: NM_001040665.1
• NCBI Official Full Name: metalloreductase STEAP2 isoform a
• NCBI Official Synonym Full Names: STEAP family member 2, metalloreductase
• NCBI Official Symbol: STEAP2
• NCBI Official Synonym Symbols: STMP; IPCA1; PUMPCn; STAMP1; PCANAP1
• NCBI Protein Information: metalloreductase STEAP2
• UniProt Protein Name: Metalloreductase STEAP2
• Protein Family: Metalloreductase
• UniProt Gene Name: STEAP2
• UniProt Synonym Gene Names: PCANAP1; STAMP1; PUMPCn
• UniProt Entry Name: STEA2_HUMAN

NCBI Description

This gene is a member of the STEAP family and encodes a multi-pass membrane protein that localizes to the Golgi complex, the plasma membrane, and the vesicular tubular structures in the cytosol. A highly similar protein in mouse has both ferrireductase and cupric reductase activity, and stimulates the cellular uptake of both iron and copper in vitro. Increased transcriptional expression of the human gene is associated with prostate cancer progression. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

STEAP2: Metalloreductase that has the ability to reduce both Fe(3+) to Fe(2+) and Cu(2+) to Cu(1+). Uses NAD(+) as acceptor. Belongs to the STEAP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Oxidoreductase; Vesicle; EC 1.16.1.-

Chromosomal Location of Human Ortholog: 7q21.13

Cellular Component: trans-Golgi network transport vesicle; intracellular membrane-bound organelle; early endosome; plasma membrane; integral to Golgi membrane; endosome membrane; cytosol

Molecular Function: transporter activity; metal ion binding; cupric reductase activity

Biological Process: regulated secretory pathway; Golgi to plasma membrane transport; iron ion homeostasis; response to hormone stimulus; copper ion import; endocytosis