Rabbit anti-Human, Mouse STEA2 Polyclonal Antibody | anti-STEA2 antibody

STEA2, ID (STEAP2, PCANAP1, STAMP1, Metalloreductase STEAP2, Prostate cancer-associated protein 1, Protein up-regulated in metastatic prostate cancer, Six-transmembrane epithelial antigen of prostate 2, SixTransMembrane protein of prostate 1) (AP)

Cat. Num. AAA6351917

• Gene Names: STEAP2; STMP; IPCA1; PUMPCn; STAMP1; PCANAP1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: STEA2; Polyclonal Antibody; ID (STEAP2; PCANAP1; STAMP1; Metalloreductase STEAP2; Prostate cancer-associated protein 1; Protein up-regulated in metastatic prostate cancer; Six-transmembrane epithelial antigen of prostate 2; SixTransMembrane protein of prostate 1) (AP); STEAP2; SixTransMembrane protein of prostate 1; anti-STEA2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human STEA2. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-STEA2 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 226-253 in the central region of human STEA2, UniProt Accession #Q8NFT2.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of 293 cell line and mouse spleen, liver tissue lysates using MBS6002447 (15ug/lane). This demonstrates that MBS6002447 detected the STEA2 protein (arrow).)

Western Blot (WB)

(Western blot analysis of lysates from 293, HepG2 cell line, mouse spleen tissue lysate (from left to right), using MBS6002447 (1:1000/lane). A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug/lane.)

Western Blot (WB)

(Western blot analysis of extracts from 293 (lanes 1 and 2), HepG2 (lanes 3 and 4) cell line and mouse spleen (lanes 5 and 6) tissue lysate: 1, 3, 5. using MBS6002447 (1:500). 2, 4, 6. usingMBS6002447, preincubated with the control peptide antigen.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma usimg MBS6002447 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Related Product Information for anti-STEA2 antibody

This gene is a member of the STEAP family and encodes a multi-pass membrane protein that localizes to the Golgi complex, the plasma membrane, and the vesicular tubular structures in the cytosol. A highly similar protein in mouse has both ferrireductase and cupric reductase activity, and stimulates the cellular uptake of both iron and copper in vitro. Increased transcriptional expression of the human gene is associated with prostate cancer progression. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Product Categories/Family for anti-STEA2 antibody

Antibodies; Transmembrane Proteins

References

1. Vaghjiani, R.J., et al. Tissue Eng Part A 15(8):2073-2083(2009). 2. Denoeud, F., et al. Genome Res. 17(6):746-759(2007). 3. Ohgami, R.S., et al. Blood 108(4):1388-1394(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 100913198
• NCBI GeneID: 261729
• NCBI Accession #: NP_001035756.1
• NCBI GenBank Nucleotide #: NM_001040666.1
• UniProt Accession #: Q8NFT2; Q6UXN6; Q6YPB1; Q8IUE7; A4D1F1; G5E9C6
• Molecular Weight: 47,916 Da
• NCBI Official Full Name: metalloreductase STEAP2 isoform b
• NCBI Official Synonym Full Names: STEAP2 metalloreductase
• NCBI Official Symbol: STEAP2
• NCBI Official Synonym Symbols: STMP; IPCA1; PUMPCn; STAMP1; PCANAP1
• NCBI Protein Information: metalloreductase STEAP2
• UniProt Protein Name: Metalloreductase STEAP2
• UniProt Gene Name: STEAP2
• UniProt Synonym Gene Names: PCANAP1; STAMP1; PUMPCn
• UniProt Entry Name: STEA2_HUMAN

NCBI Description

This gene is a member of the STEAP family and encodes a multi-pass membrane protein that localizes to the Golgi complex, the plasma membrane, and the vesicular tubular structures in the cytosol. A highly similar protein in mouse has both ferrireductase and cupric reductase activity, and stimulates the cellular uptake of both iron and copper in vitro. Increased transcriptional expression of the human gene is associated with prostate cancer progression. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

STEAP2: Metalloreductase that has the ability to reduce both Fe(3+) to Fe(2+) and Cu(2+) to Cu(1+). Uses NAD(+) as acceptor. Belongs to the STEAP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; EC 1.16.1.-; Membrane protein, integral; Vesicle; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7q21.13

Cellular Component: trans-Golgi network transport vesicle; intracellular membrane-bound organelle; early endosome; plasma membrane; integral to Golgi membrane; endosome membrane; cytosol

Molecular Function: transporter activity; metal ion binding; cupric reductase activity

Biological Process: regulated secretory pathway; Golgi to plasma membrane transport; iron ion homeostasis; response to hormone stimulus; copper ion import; endocytosis