Western Blot (WB)
(Western blot analysis of extracts from various cells using RBPSUH Antibody #MBS648482.)
Rabbit Recombining Binding Protein Suppressor of Hairless Polyclonal Antibody | anti-RBPJ antibody
Recombining Binding Protein Suppressor of Hairless (RBPSUH, CBF1, CBF-1, csl, IGKJRB, IGKJRB1, KBF2, J kappa-recombination Signal-binding Protein, RBP-J kappa, RBPJK, RBP-JK, RBPJ, RBP-J, Renal Carcinoma Antigen NY-REN-30, SUH)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
Western Blot (WB)
(Western blot analysis of extracts from various cells using RBPSUH Antibody #MBS648482.)
Western Blot (WB)
(Western blot analysis of extracts from various cells using RBPSUH Antibody #MBS648482.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a transcriptional regulator important in the Notch signaling pathway. The encoded protein acts as a repressor when not bound to Notch proteins and an activator when bound to Notch proteins. It is thought to function by recruiting chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins to Notch signaling pathway genes. Several transcript variants encoding different isoforms have been found for this gene, and several pseudogenes of this gene exist on chromosome 9. [provided by RefSeq, Oct 2013]
Uniprot Description
Function: Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA. Ref.13
Subunit structure: Interacts with activated NOTCH1, NOTCH2 or NOTCH3. Interacts with MINT/SHARP. This interaction may mediate the recruitment of large corepressor complexes containing proteins such as HDAC1, HDAC2, NCOR2, SAP30, FHL1/KYOT2 and CIR1. Interacts with EP300, MAML1 and PTF1A. Interacts with Epstein-Barr virus EBNA2, EBNA3, EBNA4 and EBNA6. Interacts with RITA/C12orf52, leading to nuclear export, prevent the interaction between RBPJ and NICD product and subsequent down-regulation of the Notch signaling pathway. Interacts with SNW1. Ref.5 Ref.6 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12
Subcellular location: Nucleus. Cytoplasm. Note: Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm, down-regulating the Notch signaling pathway. Ref.8 Ref.12
Involvement in disease: Adams-Oliver syndrome 3 (AOS3) [MIM:614814]: An autosomal dominant form of Adams-Oliver syndrome, a disorder characterized by the congenital absence of skin (aplasia cutis congenita) in combination with transverse limb defects. Aplasia cutis congenita can be located anywhere on the body, but in the vast majority of the cases, it is present on the posterior parietal region where it is often associated with an underlying defect of the parietal bones. Limb abnormalities are typically limb truncation defects affecting the distal phalanges or entire digits (true ectrodactyly). Only rarely, metatarsals/metacarpals or more proximal limb structures are also affected. Apart from transverse limb defects, syndactyly, most commonly of second and third toes, can also be observed. The clinical features are highly variable and can also include cardiovascular malformations, brain abnormalities and vascular defects such as cutis marmorata and dilated scalp veins. AOS3 patients manifest characteristic vertex scalp defects and terminal limb defects, but without congenital heart defects, other associated defects, or immune defects.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.15
Sequence similarities: Belongs to the Su(H) family.Contains 1 IPT/TIG domain.
Caution: Despite some similarity with the "phage" integrase family, it has no recombinase activity.
Sequence caution: The sequence AAA16254.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
Research Articles on RBPJ
Similar Products
Product Notes
The RBPJ rbpj (Catalog #AAA648482) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombining Binding Protein Suppressor of Hairless (RBPSUH, CBF1, CBF-1, csl, IGKJRB, IGKJRB1, KBF2, J kappa-recombination Signal-binding Protein, RBP-J kappa, RBPJK, RBP-JK, RBPJ, RBP-J, Renal Carcinoma Antigen NY-REN-30, SUH) reacts with Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Recombining Binding Protein Suppressor of Hairless can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight. Researchers should empirically determine the suitability of the RBPJ rbpj for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Recombining Binding Protein Suppressor of Hairless, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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