Western Blot (WB)
(Sample Data: Western blots from HeLa cells using anti-phospho- phospho-GSK3 beta (Ser9) antibody.)
Rabbit GSK3 beta Polyclonal Antibody | anti-phospho-GSK3beta antibody
Anti-phospho-GSK3 beta (Ser9)
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity chromatography
Synonyms
GSK3 beta; Polyclonal Antibody; Anti-phospho-GSK3 beta (Ser9); anti-phospho-GSK3beta antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the GSK3 protein phosphorylated at serine 9
Purity/Purification
Affinity chromatography
Form/Format
PBS (pH7.4) 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applicable Applications for anti-phospho-GSK3beta antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:100
IHC: 1:50-1:100
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser9
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Western Blot (WB)
(Sample Data: Western blots from HeLa cells using anti-phospho- phospho-GSK3 beta (Ser9) antibody.)
Western Blot (WB)
(Sample Data: Western blots from HeLa cells using anti-phospho- phospho-GSK3 beta (Ser9) antibody.)
Immunohistochemistry (IHC)
(Sample Data: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-phospho-GSK3 beta (Ser9) antibody.)
Immunohistochemistry (IHC)
(Sample Data: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-phospho-GSK3 beta (Ser9) antibody.)
Related Product Information for anti-phospho-GSK3beta antibody
STAT3 is a member of the signal transducers and activators of transcription (STAT) family of proteins that carry out dual functions. STAT3 is a DNA binding protein that is widely expressed and becomes activated upon phosphorylation on Tyr708, which leads dimerization, intracellular translocation and DNA-binding. Phosphorylation at Ser727 regulates transcriptional activity. STAT3 is activated in response to variety of stimuli, which includes EGF, IL-6, PDGF, IL-2 and G-CSF (1). STAT3 forms homo-and hetero-dimers with other STAT proteins to modulate the transcription of various genes (2).
Product Categories/Family for anti-phospho-GSK3beta antibody
References
1. Zhong, Z. et al: Stat3: a STAT family member activated by tyrosine phosphorylation in response to epidermal growth factor and interleukin-6. Science. 1994 Apr 1;264(5155):95-8.
2. Tian, S. S. et al: Rapid activation of the STAT3 transcription factor by granulocyte colony-stimulating factor. Blood. 1994 Sep 15;84(6):1760.
2. Tian, S. S. et al: Rapid activation of the STAT3 transcription factor by granulocyte colony-stimulating factor. Blood. 1994 Sep 15;84(6):1760.
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Product Notes
The phospho-GSK3beta (Catalog #AAA515974) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-phospho-GSK3 beta (Ser9) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GSK3 beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:100. Researchers should empirically determine the suitability of the phospho-GSK3beta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GSK3 beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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