STAT3 recombinant protein

STAT3 recombinant protein

Cat. Num. AAA515162

• Gene Names: STAT3; APRF; HIES
• Applications: Western Blot
• Purity: >95%
• Synonyms: STAT3; STAT3 recombinant protein; APRF; FLJ20882; MGC16063

• Host: Sf9 insect cells
• Purity/Purification: >95%
• Form/Format: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Concentration: 0.2 ug/ul (varies by lot)
• Sequence Length: 4978

• Applicable Applications for STAT3 recombinant protein: Kinase Assay, Western Blot (WB)
• Preparation and Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.; ; Ships in dry ice.; ; Stability: 1 year at -70°C from date of shipment.

SDS-PAGE

(The purity was determined to be >95% by densitometry. Approximately MW 120kDa)

Related Product Information for STAT3 recombinant protein

Recombinant full-length human STAT3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Scientific Background: STAT3 is a member of the signal transducers and activators of transcription (STAT) family of proteins that carry out a dual function: signal transduction and activation of transcription. STAT3 is widely expressed and becomes activated through phosphorylation on tyrosine as a DNA binding protein in response to a variety of stimuli such as EGF, IL-6, PDGF, IL-2 and G-CSF (1). This phosphoprotein forms homodimers as well as heterodimers with other members of the STAT family and translocate to the nucleus in order to modulate the transcription of various genes (2).

Product Categories/Family for STAT3 recombinant protein

Transcription Proteins; Signaling Proteins - Transcription Proteins

References

1. Zhong, Z. et al: Stat3: a STAT family member activated by tyrosine phosphorylation in response to epidermal growth factor and interleukin-6. Science. 1994 Apr 1;264(5155):95-8.
2. Tian, S. S. et al: Rapid activation of the STAT3 transcription factor by granulocyte colony-stimulating factor. Blood. 1994 Sep 15;84(6):1760

NCBI and Uniprot Product Information

• NCBI GI #: 47080104
• NCBI GeneID: 6774
• NCBI Accession #: NP_644805.1
• NCBI GenBank Nucleotide #: NM_139276
• UniProt Accession #: P40763; O14916; Q9BW54; A8K7B8
• Molecular Weight: ~120 kDa
• NCBI Official Full Name: Homo sapiens signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 3 (acute-phase response factor)
• NCBI Official Symbol: STAT3
• NCBI Official Synonym Symbols: APRF; HIES
• NCBI Protein Information: signal transducer and activator of transcription 3; DNA-binding protein APRF; acute-phase response factor
• UniProt Protein Name: Signal transducer and activator of transcription 3
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: STAT3
• UniProt Synonym Gene Names: APRF
• UniProt Entry Name: STAT3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity. Plays an important role in host defense in methicillin-resistant S.aureus lung infection by regulating the expression of the antimicrobial lectin REG3G

By similarity. Ref.9 Ref.10 Ref.14 Ref.17 Ref.39

Subunit structure: Forms a homodimer or a heterodimer with a related family member (at least STAT1). Interacts with IL31RA, NCOA1, PELP1, SIPAR, SOCS7, STATIP1 and TMF1. Interacts with HCV core protein. Interacts with IL23R in presence of IL23. Interacts (via SH2 domain) with NLK. Interacts with ARL2BP; the interaction is enhanced by LIF and JAK1 expression

By similarity. Interacts with KPNA4 and KPNA5; KPNA4 may be the primary mediator of nuclear import

By similarity. Interacts with CAV2; the interaction is increased on insulin-induced tyrosine phosphorylation of CAV2 and leads to STAT3 activation

By similarity. Interacts with ARL2BP; interaction is enhanced with ARL2. Interacts with NEK6

By similarity. Binds to CDK9 when activated and nuclear. Interacts with BMX. Interacts with ZIPK/DAPK3. Interacts with PIAS3; the interaction occurs on stimulation by IL6, CNTF or OSM and inhibits the DNA binding activity of STAT3. In prostate cancer cells, interacts with STAT3 and promotes DNA binding activity of STAT3. Interacts with STMN3, antagonizing its microtubule-destabilizing activity. Interacts with the 'Lys-129' acetylated form of BIRC5/survivin. Interacts with FER. Interacts (via SH2 domain) with EIF2AK2/PKR (via the kinase catalytic domain). Ref.8 Ref.9 Ref.11 Ref.12 Ref.13 Ref.14 Ref.17 Ref.19 Ref.20 Ref.21 Ref.22 Ref.25 Ref.27 Ref.29 Ref.30 Ref.34 Ref.35 Ref.39

Subcellular location: Cytoplasm. Nucleus. Note: Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1. Ref.21 Ref.26 Ref.30

Tissue specificity: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Post-translational modification: Tyrosine phosphorylated upon stimulation with EGF. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4

By similarity. Activated through tyrosine phosphorylation by BMX. Tyrosine phosphorylated in response to IL6, IL11, LIF, CNTF, KITLG/SCF, CSF1, EGF, PDGF, IFN-alpha and OSM. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus. Upon LPS challenge, phosphorylated within the nucleus by IRAK1. Upon erythropoietin treatment, phosphorylated on Ser-727 by RPS6KA5. Phosphoryation at Tyr-705 by PTK6 or FER leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates IL6/interleukin-6 signaling. Ref.7 Ref.9 Ref.10 Ref.15 Ref.18 Ref.21 Ref.24 Ref.25 Ref.28 Ref.30 Ref.34 Ref.38

Involvement in disease: Hyperimmunoglobulin E recurrent infection syndrome, autosomal dominant (AD-HIES) [MIM:147060]: A rare disorder of immunity and connective tissue characterized by immunodeficiency, chronic eczema, recurrent Staphylococcal infections, increased serum IgE, eosinophilia, distinctive coarse facial appearance, abnormal dentition, hyperextensibility of the joints, and bone fractures.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.40 Ref.41

Miscellaneous: Involved in the gp130-mediated signaling pathway.

Sequence similarities: Belongs to the transcription factor STAT family.Contains 1 SH2 domain.