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Immunoprecipitation (IP) (Sample Data: Representative Immunoprecipitation with 2 ug of Anti-VAV using 500 ug of Jurkat and THP1cell lysates.)

Rabbit VAV Polyclonal Antibody | anti-VAV antibody

Anti-VAV

Reactivity
Western blot VAV from human cells
VAV from other species may also be detectable.
Applications
Western Blot, Immunoprecipitation
Purity
Affinity chromatography
Synonyms
VAV; Polyclonal Antibody; Anti-VAV; anti-VAV antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Western blot VAV from human cells
VAV from other species may also be detectable.
Clonality
Polyclonal
Specificity
Recognizes the VAV protein
Purity/Purification
Affinity chromatography
Form/Format
PBS, pH7.4, 0.02% NaN3, 50% glycerol
Applicable Applications for anti-VAV antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 18003:19002
IP: 2ug
Immunogen
Synthetic peptide corresponding to amino acids 172-176 of human VAV
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Immunoprecipitation (IP)

(Sample Data: Representative Immunoprecipitation with 2 ug of Anti-VAV using 500 ug of Jurkat and THP1cell lysates.)

Immunoprecipitation (IP) (Sample Data: Representative Immunoprecipitation with 2 ug of Anti-VAV using 500 ug of Jurkat and THP1cell lysates.)

Western Blot (WB)

(Sample Data: Representative Western blot with Anti-VAV (1:1000) using 3 ug, 10 ug and 30 ug of Jurkat cell lysate.)

Western Blot (WB) (Sample Data: Representative Western blot with Anti-VAV (1:1000) using 3 ug, 10 ug and 30 ug of Jurkat cell lysate.)
Related Product Information for anti-VAV antibody
VAV is a proto-oncogene that is a member of the Dbl family of guanine nucleotide exchange factors (GEF) for the Rho family of GTP binding proteins. VAV plays a vital role in hematopoiesis and in T-cell and B-cell development as well as in their activation. VAV is the specific binding partner of Nef proteins from HIV-1 (1). Co-expression and binding of these VAV partners initiates profound morphological changes, cytoskeletal rearrangements and JNK/SAPK pathway activation leading to increased levels of viral transcription and replication. VAV is also the guanine nucleotide dissociation inhibitor for Ras.
References
1. Fackler, O T. et al: Activation of Vav by Nef induces cytoskeletal rearrangements and downstream effector functions. Molec. Cell 3729-739, 1999.

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Product Notes

The VAV (Catalog #AAA516298) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-VAV reacts with Western blot VAV from human cells VAV from other species may also be detectable. and may cross-react with other species as described in the data sheet. AAA Biotech's VAV can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 18003:19002 IP: 2ug. Researchers should empirically determine the suitability of the VAV for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VAV, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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