Western Blot (WB)
((1ug/ml) staining of Human Colorectal cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat PYROXD1 Polyclonal Antibody | anti-PYROXD1 antibody
Goat anti-PYROXD1 Antibody
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
PYROXD1; Polyclonal Antibody; Goat anti-PYROXD1 Antibody; pyridine nucleotide-disulphide oxidoreductase domain 1; FLJ22028; OTTHUMP00000240138; OTTHUMP00000240140; pyridine nucleotide-disulfide oxidoreductase domain-containing protein 1; FLJ22028 antibody; OTTHUMP00000240138 antibody; OTTHUMP00000240140 antibody; pyridine nucleotide-disulfide oxidoreductase domain-containing protein 1 antibody; pyridine nucleotide-disulphide oxidoreductase domain 1 antibody; PYROXD1 antibody; anti-PYROXD1 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
TCAEQLATHFPSED
Sequence Length
500
Applicable Applications for anti-PYROXD1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 55+48kDa bands observed in Human Colorectal cancer lysates (calculated MW of 55.8kDa according to NP_079130.2). The observed 48kDa band corresponds to earlier findings with different antibodies from other commercial sources and likely represents a breakdown product. Recommended concentration: 1-3ug/ml.
Western Blot: Approx 55+48kDa bands observed in Human Colorectal cancer lysates (calculated MW of 55.8kDa according to NP_079130.2). The observed 48kDa band corresponds to earlier findings with different antibodies from other commercial sources and likely represents a breakdown product. Recommended concentration: 1-3ug/ml.
Immunogen
Peptide with sequence TCAEQLATHFPSED, from the N Terminus of the protein sequence according to NP_079130.2.
Epitope
N Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((1ug/ml) staining of Human Colorectal cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Human Colorectal cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,338 Da
NCBI Official Full Name
pyridine nucleotide-disulfide oxidoreductase domain-containing protein 1
NCBI Official Synonym Full Names
pyridine nucleotide-disulphide oxidoreductase domain 1
NCBI Official Symbol
PYROXD1
NCBI Protein Information
pyridine nucleotide-disulfide oxidoreductase domain-containing protein 1
UniProt Protein Name
Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 1
UniProt Gene Name
PYROXD1
UniProt Entry Name
PYRD1_HUMAN
Uniprot Description
PYROXD1: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. PYROXD1 subfamily.
Protein type: Oxidoreductase; EC 1.8.1.-
Chromosomal Location of Human Ortholog: 12p12.1
Molecular Function: oxidoreductase activity; protein binding
Research Articles on PYROXD1
Similar Products
Product Notes
The PYROXD1 pyroxd1 (Catalog #AAA423064) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-PYROXD1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's PYROXD1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 55+48kDa bands observed in Human Colorectal cancer lysates (calculated MW of 55.8kDa according to NP_079130.2). The observed 48kDa band corresponds to earlier findings with different antibodies from other commercial sources and likely represents a breakdown product. Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the PYROXD1 pyroxd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: TCAEQLATHF PSED. It is sometimes possible for the material contained within the vial of "PYROXD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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