Western Blot (WB)
(Western Blot (WB) analysis of SRp46 (E72) pAb at 1:500 dilutionLane1:PC12 whole cell lysate (40ug) Lane2:The Embryo tissue lysate of Mouse (40ug) Lane3:EC9706 whole cell lysate (20ug) Lane4:HEK293T whole cell lysate (10ug) Lane5:A549 whole cell lysate (20ug))
Rabbit SRp46 Polyclonal Antibody | anti-SRp46 antibody
SRp46 (E72) Polyclonal Antibody
Gene Names
SRSF8; DSM-1; SRP46; SFRS2B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
SRp46; Polyclonal Antibody; SRp46 (E72) Polyclonal Antibody; arginine/serine-rich 2B; Pre mRNA splicing factor SRP46; Pre-mRNA-splicing factor SRP46; Serine/arginine-rich splicing factor 8; SFRS2B; Splicing factor; Splicing factor arginine serine rich 46kD; Splicing factor arginine serine rich 2B; Splicing factor SRp46; SRP46; SRSF8; anti-SRp46 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SRp46 (E72) polyclonal antibody detects endogenous levels of SRp46 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
282
Applicable Applications for anti-SRp46 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Synthetic peptide, corresponding to amino acids 50-100 of Human SRp46.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of SRp46 (E72) pAb at 1:500 dilutionLane1:PC12 whole cell lysate (40ug) Lane2:The Embryo tissue lysate of Mouse (40ug) Lane3:EC9706 whole cell lysate (20ug) Lane4:HEK293T whole cell lysate (10ug) Lane5:A549 whole cell lysate (20ug))
Western Blot (WB)
(Western Blot (WB) analysis of SRp46 (E72) pAb at 1:500 dilutionLane1:PC12 whole cell lysate (40ug) Lane2:The Embryo tissue lysate of Mouse (40ug) Lane3:EC9706 whole cell lysate (20ug) Lane4:HEK293T whole cell lysate (10ug) Lane5:A549 whole cell lysate (20ug))
Related Product Information for anti-SRp46 antibody
The family of SR factors all contain one or more RNA recognition motifs (RRM) and an arginine/serine (RS)-rich domain, are essential for constitutive splicing and also regulate splicing in a concentration-dependent manner by influencing the selection of alternative splice sites. The majority of SR proteins, including SC35 and SRp40, are confined to the nucleus, while SF2/ASF, SRp20, and 9G8 are continuously shuttled between the nucleus and the cytoplasm and contribute to mRNA transport. The activity of SR proteins in regulated splicing is antagonized by members of the hnRNP A/B family of proteins, which induce drastic shifts in the selection of splicing-sites. An additional SR-associated protein, p32, tightly associates with SR factors and preferentially inhibits ASF/SF2 functioning as both a splicing enhancer and splicing repressor protein by preventing the stable interaction of ASF/SF2 and the RNA.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 35kDa
NCBI Official Full Name
serine/arginine-rich splicing factor 8
NCBI Official Synonym Full Names
serine and arginine rich splicing factor 8
NCBI Official Symbol
SRSF8
NCBI Official Synonym Symbols
DSM-1; SRP46; SFRS2B
NCBI Protein Information
serine/arginine-rich splicing factor 8
UniProt Protein Name
Serine/arginine-rich splicing factor 8
UniProt Gene Name
SRSF8
UniProt Synonym Gene Names
SFRS2B; SRP46; Splicing factor SRp46
UniProt Entry Name
SRSF8_HUMAN
NCBI Description
This gene encodes a member of a family of proteins containing a ribonucleoprotein (RNP)-type RNA binding motif and a carboxyl-terminal arginine-serine-rich (RS) domain. The encoded protein functions as a pre-mRNA splicing factor. There is a pseudogene for this gene on chromosome 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
Uniprot Description
SRp46: Involved in pre-mRNA alternative splicing. Belongs to the splicing factor SR family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA-binding
Chromosomal Location of Human Ortholog: 11q22
Cellular Component: nucleus
Molecular Function: protein binding; nucleotide binding
Biological Process: RNA splicing; mRNA processing
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Product Notes
The SRp46 srsf8 (Catalog #AAA3013745) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SRp46 (E72) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SRp46 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the SRp46 srsf8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SRp46, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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